Genome of Sindbis virus.

Journal of virology ·Vol. 9 ·No. 1 ·1972-01-00 ·Pages 102-9

Arif BM, Faulkner P

Abstract

(32)P-labeled ribonucleic acid (RNA) from purified Sindbis virus was examined for the presence of hidden breaks. Viral RNA was treated with acid at pH 2.9 or with formaldehyde and was analyzed on sucrose gradients or by polyacrylamide gel electrophoresis. The sedimentation pattern and mobility on polyacrylamide gels of the 42S RNA was unaffected by heating and quick cooling and was not altered by denaturing agents such as dimethyl sulfoxide and urea. No evidence that Sindbis RNA is a polyaggregate of fragments was obtained. It is concluded that the genome consists of a continuous length of single-stranded polynucleotide.

MeSH Terms

Animals Arboviruses/analysis Cell Line Centrifugation, Density Gradient Cesium Chlorides Cricetinae Dimethyl Sulfoxide Electrophoresis, Disc Formaldehyde Hot Temperature Hydrochloric Acid Hydrogen-Ion Concentration Kidney Molecular Weight Phosphorus Isotopes Polynucleotides/analysis RNA, Viral/analysis,isolation & purification Sindbis Virus/analysis,growth & development,isolation & purification Sucrose Urea Virus Cultivation

Chemicals

Chlorides Phosphorus Isotopes Polynucleotides RNA, Viral Formaldehyde Cesium Sucrose Urea Hydrochloric Acid Dimethyl Sulfoxide

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Arif B M

Faulkner P

References (16)

16 references, click to expand

Differences between the structures of poliovirus and Sindbis virus infectious ribonucleic acids.
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A kinetic analysis of the synthesis in BHK 21 cells of RNAs specific for Semliki Forest virus.
J Gen Virol. 1969 Sep;5(2):251-65 PMID: 5388361
Visualisation in the electron microscope and size of RNA from animal cells.
Eur J Biochem. 1969 May 1;9(1):1-20 PMID: 4306663
The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.
Biochem J. 1969 Jun;113(1):131-8 PMID: 4896715
Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
Denaturation of Sindbis virus RNA with dimethyl sulphoxide.
J Gen Virol. 1971 Jan;10(1):103-6 PMID: 5542484
The effect of reaction with formaldehyde on the sedimentation rates of ribonucleic acids.
Biochem J. 1968 May;107(6):851-9 PMID: 16742611
Properties of 42S and 26S Sindbis viral ribonucleic acid species.
J Virol. 1969 Oct;4(4):429-38 PMID: 5823232
Characterization of a cytoplasmic fraction from Sindbis virus-infected cultures.
Can J Microbiol. 1969 Feb;15(2):215-22 PMID: 5764278
Molecular weight of Sindbis virus ribonucleic acid as measured by polyacrylamide gel electrophoresis.
J Virol. 1970 Jul;6(1):145-7 PMID: 5471476
Release of viral hemagglutinin and nucleocapsid components after disruption of Sindbis virus with ether.
Can J Microbiol. 1971 Feb;17(2):161-9 PMID: 5102213
The reaction of ribonucleic acid with formaldehyde. I. Optical absorbance studies.
Biochemistry. 1967 Sep;6(9):2718-27 PMID: 4861595
Denaturation of RNA with dimethyl sulfoxide.
Biopolymers. 1968 Jun;6(6):793-807 PMID: 5654613
Virus nucleic acids formed in chick embryo cells infected with Semliki Forest virus.
J Gen Virol. 1970 Feb;6(2):231-48 PMID: 5461804
Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses.
Am J Trop Med Hyg. 1958 Sep;7(5):561-73 PMID: 13571577
Replication of Western equine encephalomyelitis virus. I. Some chemical and physical characteristics of viral ribonucleic acid.
J Virol. 1968 Jun;2(6):558-66 PMID: 5697470

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.9.1.102-109.1972

Published

1972-01-00

Pages

102-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356268

Subset

External Links

PubMed source DOI: 10.1128/JVI.9.1.102-109.1972 PMC Full Text View journal details

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