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PMID: 453323 Published · ppublish English Journal Article

Neutrophil aggregation and degranulation. Effect of arachidonic acid.

The American journal of pathology ·Vol. 95 ·No. 2 ·1979-05-00 ·Pages 433-44

O'Flaherty JT, Showell HJ, Becker EL, Ward PA

Abstract

In response to aggregating and degranulating stimuli, platelets metabolize endogenous arachidonic acid to bioactive derivatives. These derivatives can stimulate platelets to degranulate and aggregate and, therefore, may be mediators of the platelet response. Because exogenous arachidonic acid also stimulates platelets to degranulate, aggregate, and form these mediators, we examined the effect of adding arachidonic acid to purified human neutrophil suspensions. Micromolar concentrations of arachidonic acid stimulated neutrophils to aggregate but not to degranulate. Cytochalasin B, a potentiator of neutrophil responses to chemotactic factors, also potentiated the arachidonic-acid-induced aggregation response; 5,8,11,14-eicosatetraynoic acid, an inhibitor of arachidonic acid metabolism, blocked this response. Aggregation of neutrophils, was not stimulated by several fatty acids with structural similarity to arachidonic acid. These results suggest that metabolic derivatives of arachidonic acid may be active in stimulating certain neutrophil responses. The role of these derivatives in mediating neutrophil responses to various stimuli needs to be examined.

MeSH Terms
5,8,11,14-Eicosatetraynoic Acid/pharmacology Arachidonic Acids/pharmacology Cell Aggregation/drug effects Cytochalasin B/pharmacology Eicosanoic Acids/pharmacology Humans L-Lactate Dehydrogenase/metabolism Lysosomes/drug effects Muramidase/metabolism Neutrophils/drug effects,physiology Platelet Aggregation/drug effects Structure-Activity Relationship
Chemicals
Arachidonic Acids Eicosanoic Acids 5,8,11,14-Eicosatetraynoic Acid Cytochalasin B L-Lactate Dehydrogenase Muramidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
O'Flaherty J T
Showell H J
Becker E L
Ward P A
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1979-05-00
Pages
433-44
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC2042334
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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