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PMID: 830662 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stimulation of human eosinophil and neutrophil polymorphonuclear leukocyte chemotaxis and random migration by 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid.

The Journal of clinical investigation ·Vol. 59 ·No. 1 ·1977-01-00 ·Pages 179-83

Goetzl EJ, Woods JM, Gorman RR

Abstract

A human platelet lipoxygenase-generated product of arachidonic acid, identified by thin-layer chromatographic and mass spectrometric properties as 12-L-hydroxy-5,8,10,14-eicosatertraenoic acid (HETE), was selectively chemotactic in vitro for human polymorphonuclear leukocytes (PMN), as compared to mononuclear leukocytes, with a preference for eosinophils. Preincubation of PMN with partially-purified HETE at peak chemotactic concentrations of 8-24 mug/ml reduced their random and chemotactic migration and stimulated the activity of their hexose monophosphate shunt; minimally chemotactic concentrations of 0.03-1 mug/ml enhanced PMN random migration without influencing other functions. HETE may thus be capable of preferentially attracting eosinophils to foci of tissue reaction associated with platelet activation.

MeSH Terms
Arachidonic Acids/pharmacology Cell Movement/drug effects Chemotaxis/drug effects Dose-Response Relationship, Drug Eosinophils/physiology Humans Neutrophils/physiology
Chemicals
Arachidonic Acids
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goetzl E J
Woods J M
Gorman R R
References (18)
18 references, click to expand
  1. ISOLATION OF BETA IF-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT.
    J Exp Med. 1965 Aug 1;122:277-98 PMID: 14316946
  2. Stimulation of human neutrophil leukocyte aerobic glucose metabolism by purified chemotactic factors.
    J Clin Invest. 1974 Feb;53(2):591-9 PMID: 11344574
  3. A neutrophil-immobilizing factor derived from human leukocytes. I. Generation and partial characterization.
    J Exp Med. 1972 Dec 1;136(6):1564-80 PMID: 4641855
  4. The IgE-mediated release of an eosinophil leukocyte chemotactic factor from human lung.
    J Immunol. 1971 Sep;107(3):899-902 PMID: 4105937
  5. Plasma and cell-derived inhibitors of human neutrophil chemotaxis.
    Ann N Y Acad Sci. 1975 Jun 13;256:210-21 PMID: 1099960
  6. Prostaglandin endoperoxides. Novel transformations of arachidonic acid in human platelets.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3400-4 PMID: 4215079
  7. Modulation of the random migration of human platelets.
    J Clin Invest. 1974 Nov;54(5):1100-6 PMID: 4608469
  8. Modulation of human neutrophil polymorphonuclear leucocyte migration by human plasma alpha-globulin inhibitors and synthetic esterase inhibitors.
    Immunology. 1975 Jul;29(1):163-74 PMID: 49293
  9. Prostaglandin endoperoxides. A new concept concerning the mode of action and release of prostaglandins.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):3824-8 PMID: 4530264
  10. Biogenesis of chemotactic molecules by the arachidonate lipoxygenase system of platelets.
    Nature. 1975 Oct 23;257(5528):680-1 PMID: 810723
  11. Arachidonate lipoxygenase in blood platelets.
    Biochim Biophys Acta. 1975 Feb 20;380(2):299-307 PMID: 804329
  12. New aspects of chemotaxis. Specific target-cell attraction by lipid and lipoprotein fractions of Escherichia coli chemotactic factor.
    Am J Pathol. 1975 Nov;81(2):401-10 PMID: 1103633
  13. Purification and synthesis of eosinophilotactic tetrapeptides of human lung tissue: identification as eosinophil chemotactic factor of anaphylaxis.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):4123-7 PMID: 1060093
  14. Prostaglandins and chemotaxis: enhancement of polymorphonuclear leukocyte chemotaxis by prostaglandin F2alpha.
    J Invest Dermatol. 1976 Mar;66(3):149-52 PMID: 1249443
  15. Chemotactic deactivation of human eosinophils by the eosinophil chemotactic factor of anaphylaxis (38527).
    Proc Soc Exp Biol Med. 1975 Jan;148(1):301-6 PMID: 1129271
  16. Polymorphonulcear leukocyte chemotaxis toward oxidized lipid components of cell membranes.
    J Exp Med. 1975 Jun 1;141(6):1437-41 PMID: 1127383
  17. The deactivation of rabbit neutrophils by chemotactic factor and the nature of the activatable esterase.
    J Exp Med. 1968 Apr 1;127(4):693-709 PMID: 5642465
  18. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes.
    J Exp Med. 1962 Mar 1;115:453-66 PMID: 13872176
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108617
Published
1977-01-00
Pages
179-83
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC333346
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108617 PMC Full Text View journal details
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