The 3'-terminal nucleotide sequences of bacteriophage lambda DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 4 ·1973-04-00 ·Pages 1151-5

Weigel PH, Englund PT, Murray K, Old RW

Abstract

Analyses of radioactive oligonucleotides in endonuclease digests of 3'-terminally labeled lambda DNA revealed the 3' terminal sequence -GTTACG for the l strand and -ACCCGCG for the r strand. These sequences, together with those previously known for the 5' cohesive ends, provide a total of 25 known base-pairs in the vicinity of the termini. When the cohesive ends are paired, the sequence between the nicks can be bisected by a 2-fold rotational axis of symmetry. Five of the first eight base-pairs, on either side of the axis, are rotationally symmetric. This symmetry may be involved in the recognition of the site by an enzyme responsible for formation of the cohesive ends.

MeSH Terms

Adenine Nucleotides/analysis Base Sequence Coliphages/analysis Cytosine Nucleotides/analysis DNA, Viral/analysis Deoxyribonucleases Electrophoresis, Paper Guanine Nucleotides/analysis Hydrolysis Models, Structural Oligonucleotides/analysis Phosphorus Isotopes Polynucleotides/analysis Thymine Nucleotides/analysis

Chemicals

Adenine Nucleotides Cytosine Nucleotides DNA, Viral Guanine Nucleotides Oligonucleotides Phosphorus Isotopes Polynucleotides Thymine Nucleotides Deoxyribonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Weigel P H

Englund P T

Murray K

Old R W

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27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.4.1151

Published

1973-04-00

Pages

1151-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433446

Subset

Databases

GENBANK

J02459, M17233

External Links

PubMed source DOI: 10.1073/pnas.70.4.1151 PMC Full Text View journal details

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The 3'-terminal nucleotide sequences of bacteriophage lambda DNA.

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