Home LiteratureArticle Details
PMID: 4462590 Published · ppublish English Journal Article

Specific changes in the messenger ribonucleic acid content of the rat ventral prostate gland after androgenic stimulation. Evidence from the synthesis of aldolase messenger ribonucleic acid.

The Biochemical journal ·Vol. 144 ·No. 2 ·1974-11-00 ·Pages 413-26

Mainwaring WI, Mangan FR, Irving RA, Jones DA

Abstract

1. Aldolase was selected as a suitable marker for following the androgenic regulation of mRNA synthesis in the prostate gland. 2. Antibodies raised in rabbits against crystalline prostate aldolase were used to monitor the synthesis of this androgen-induced enzyme after hormonal stimulation of castrated animals, by using procedures in vivo and in vitro for the translation of prostate poly(A)-rich mRNA. 3. After androgenic stimulation in vivo the poly(A)-rich mRNA was isolated from the prostate gland and other tissues of castrated rats, and added to a protein-synthesizing system in vitro derived from Krebs II ascites-tumour cells. By using this approach it was found that androgens regulate the synthesis of aldolase mRNA in a highly tissue-specific manner. Stimulation of aldolase mRNA synthesis reached a maximum after 8h of androgenic treatment and then declined. 4. The androgenic control of aldolase mRNA synthesis was also investigated in vivo. After treatment of castrated animals with various steroids in vivo [(35)S]methionine was injected directly into the prostate gland, and labelled aldolase was selectively precipitated from isolated polyribosomes with anti-aldolase serum. The regulation of aldolase mRNA synthesis in the prostate gland was stringently steroid-specific and could only be evoked by androgens. After a single injection of testosterone, aldolase synthesis reached a maximum after 16h of hormonal stimulation and then declined. 5. Although androgens exert significant control over transcriptional processes in the prostate gland, and appear to regulate the synthesis of aldolase mRNA de novo, the possibility exists for additional means of control at the translational level of aldolase synthesis. The results are discussed in the context of the overall mechanism of action of androgens.

MeSH Terms
Androgens/pharmacology Animals Antibodies Castration Electrophoresis, Polyacrylamide Gel Fructose-Bisphosphate Aldolase/biosynthesis,immunology,isolation & purification Immune Sera Male Methionine/metabolism Polyribosomes/analysis Prostate/analysis,drug effects RNA, Messenger/analysis,biosynthesis Rabbits/immunology Rats Sulfur Radioisotopes Testosterone/administration & dosage,pharmacology
Chemicals
Androgens Antibodies Immune Sera RNA, Messenger Sulfur Radioisotopes Testosterone Methionine Fructose-Bisphosphate Aldolase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mainwaring W I
Mangan F R
Irving R A
Jones D A
References (58)
58 references, click to expand
  1. Experiments on hemoglobin biosynthesis.
    Proc Natl Acad Sci U S A. 1961 Jul 15;47:941-50 PMID: 13782056
  2. Studies on the microsoml sodium-plus-potassium ion-stimulated adenosine triphosphatase system in rat ventral prostate.
    Biochem J. 1969 Aug;113(5):829-36 PMID: 4241688
  3. Changes in the enzymatic constitution of the ventral prostate gland induced by androgenic hormones.
    Endocrinology. 1954 Feb;54(2):121-9 PMID: 13151104
  4. Structural relations in aldolases purified from rat liver and muscle and Novikoff hepatoma.
    Arch Biochem Biophys. 1970 Feb;136(2):480-90 PMID: 4314111
  5. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.
    Biochim Biophys Acta. 1960 Mar 11;38:470-83 PMID: 13826559
  6. Isolation of eukaryotic messenger RNA on cellulose and its translation in vitro.
    Biochem Biophys Res Commun. 1972 Nov 1;49(3):680-9 PMID: 4638747
  7. In vivo labeling patterns of free polyribosomes: relationship to tape theory of messenger ribonucleic acid function.
    J Mol Biol. 1967 Jun 14;26(2):211-25 PMID: 6034773
  8. Selective uptake of radioactivity in rat ventral prostate following administration of testosterone-1,2-3H. Methodological considerations.
    Acta Endocrinol (Copenh). 1968 Oct;59(2):218-26 PMID: 5754969
  9. The synthesis and degradation of citric acid by ventral prostate tissue. I. Enzymatic mechanisms.
    J Biol Chem. 1954 May;208(1):337-44 PMID: 13174543
  10. The translation of mouse globin mRNA from which the polyadenylic acid sequence has been removed in a reinitiating protein synthesis system.
    Biochem Biophys Res Commun. 1974 Jun 18;58(4):927-31 PMID: 4134608
  11. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  12. The identity of the aldolases isolated from rat muscle and primary hepatoma.
    Arch Biochem Biophys. 1970 Feb;136(2):491-7 PMID: 4314112
  13. INTERACTION OF RIBOSOMES AND SYNTHETIC POLYRIBONUCLEOTIDES.
    J Mol Biol. 1963 Oct;7:323-33 PMID: 14066611
  14. Protein synthesis directed by encephalomyocarditis virus RNA: properties of a transfer RNA-dependent system.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2303-7 PMID: 4332253
  15. Ovalbumin messenger ribonucleic acid translation. Comparable rates of polypeptide initiation and elongation on ovalbumin and globin messenger ribonucleic acid in a rabbit reticulocyte lysate.
    J Biol Chem. 1973 Mar 25;248(6):2095-106 PMID: 4690593
  16. Studies on the aldolase isozymes by the polyacrylamide gel disc electrophoresis.
    Comp Biochem Physiol. 1970 Feb 1;32(3):543-53 PMID: 5435732
  17. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336-40 PMID: 5288383
  18. Multiple forms of fructose diphosphate aldolase in mammalian tissues.
    Proc Natl Acad Sci U S A. 1966 Oct;56(4):1275-82 PMID: 5230152
  19. Isozyme patterns of lactate dehydrogenase, creatine phosphokinase, phosphoglucomutase and aldolase guinea pig tissues during ontogeny.
    J Exp Zool. 1972 Nov;182(2):201-10 PMID: 5079085
  20. Factors for the initiation of haemoglobin synthesis by rabbit reticulocyte ribosomes.
    Nature. 1970 May 9;226(5245):511-4 PMID: 5442342
  21. Glucose 6-phosphate dehydrogenase from brewers' yeast (Zwischenferment). 3. Studies on the subunit structure and on the molecular association phenomenon induced by triphosphopyridine nucleotide.
    J Biol Chem. 1969 Mar 10;244(5):1353-64 PMID: 4388027
  22. Autoradiographic localization of androgen in the rat ventral prostate.
    Endocrinology. 1969 Aug;85(2):350-4 PMID: 5784923
  23. Separation of two factors, M1 and M2, required for poly U dependent polypeptide synthesis by rabbit reticulocyte ribosomes at low magnesium ion concentration.
    Biochem Biophys Res Commun. 1970 Feb 20;38(4):721-7 PMID: 5443714
  24. Estrogen-induced changes in translation, and specific messenger RNA levels during oviduct differentiation.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2377-80 PMID: 4506757
  25. Increased efficiency of exogenous messenger RNA translation in a Krebs ascites cell lysate.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1299-303 PMID: 4338590
  26. The use of deoxyribonucleic acid-cellulose chromatography and isoelectric focusing for the characterization and partial purification of steroid-receptor complexes.
    Biochem J. 1973 May;134(1):113-27 PMID: 4353081
  27. Subunit structure and chemical properties of rabbit liver aldolase.
    J Biol Chem. 1969 Jul 25;244(14):3913-9 PMID: 5805405
  28. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5 PMID: 5288382
  29. Studies on the form and synthesis of messenger ribonucleic acid in the rat ventral prostate gland, including its tissue-specific stimulation by androgens.
    Biochem J. 1974 Mar;137(3):513-24 PMID: 4421176
  30. Induction of avidin synthesis by RNA obtained from chick oviduct.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2814-7 PMID: 4507605
  31. Protein biosynthesis on chick oviduct polyribosomes. II. Regulation by progesterone.
    Biochemistry. 1971 Apr 27;10(9):1570-6 PMID: 5580670
  32. Polypeptide synthesis in Escherichia coli. I. Ribosomes and the active complex.
    J Mol Biol. 1963 May;6:374-88 PMID: 13947830
  33. Ovalbumin messenger RNA of chick oviduct: partial characterization, estrogen dependence, and translation in vitro.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1146-50 PMID: 4504328
  34. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321-5 PMID: 5288381
  35. Rabbit reticulocyte ribosomes and Krebs ascites supernatant: a mixed system dependent upon added mRNA.
    Biochim Biophys Acta. 1973 Dec 7;331(2):243-50 PMID: 4774408
  36. The control of the form and function of the ribosomes in androgen-dependent tissues by testosterone.
    Biochem J. 1973 Jul;134(3):795-805 PMID: 4749277
  37. The effects of castration and androgen replacement on the nucleic acid composition, metabolism, and enzymatic capacities of the rat ventral prostate.
    Endocrinology. 1958 Sep;63(3):271-9 PMID: 13574081
  38. Isolation of a protein fraction from reticulocyte ribosomes required for de novo synthesis of hemoglobin.
    Arch Biochem Biophys. 1968 May;125(2):632-46 PMID: 5656813
  39. Separation and quantitation of lactic dehydrogenase isoenzymes by disc electrophoresis.
    Anal Biochem. 1967 Aug;20(2):246-57 PMID: 4167387
  40. Early appearance of histone messenger RNA in polyribosomes of cultured L cells.
    J Mol Biol. 1972 Feb 14;63(3):591-6 PMID: 4111381
  41. EVOLUTION OF ALDOLASE.
    Fed Proc. 1964 Nov-Dec;23:1248-57 PMID: 14236133
  42. Translation of mouse globin messenger ribonucleic acid from which the poly(adenylic acid) sequence has been removed.
    Biochemistry. 1974 Feb 12;13(4):703-7 PMID: 4129862
  43. Mammalian cell-free protein synthesis directed by viral ribonucleic acid.
    Eur J Biochem. 1970 Dec;17(2):328-38 PMID: 5500401
  44. Studies on the solubilized ribonucleic acid polymerase from rat ventral prostate gland.
    Biochem J. 1971 Jul;123(4):619-28 PMID: 5166637
  45. Molecular and catalytic properties of aldolase C.
    Biochemistry. 1969 Nov;8(11):4396-402 PMID: 4982047
  46. Synthesis of inducible tyrosine aminotransferase in a cell-free extract from cultured hepatoma cells.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3615-9 PMID: 4405028
  47. Influence of gonadectomy, sex hormones and other factors, on the activity of certain glycosidases in the rat and mouse.
    J Endocrinol. 1959 Mar;18(2):132-46 PMID: 13641550
  48. Protein biosynthesis on chick oviduct polyribosomes. I. Changes during estrogen-mediated tissue differentiation.
    Biochemistry. 1971 Apr 27;10(9):1561-70 PMID: 4931748
  49. A reconstituted cell-free system for the specific transfer of steroid--receptor complexes into nuclear chromatin isolated from the rat ventral prostate gland.
    Biochem J. 1971 Nov;125(1):285-95 PMID: 5158914
  50. Biochemical characterization of prostatic nuclei. I. Androgen-induced changes in nuclear proteins.
    Biochim Biophys Acta. 1971 Nov 19;247(4):570-83 PMID: 5141666
  51. Aldose reductase and ketose reductase in male accessory organs of reproduction. Distribution and relation to seminal fructose.
    Biochem J. 1962 Jul;84:39-45 PMID: 14496688
  52. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  53. Biogenesis and characterization of histone messenger RNA in HeLa cells.
    J Mol Biol. 1972 Jun 28;67(3):397-406 PMID: 5045304
  54. The determination of nucleic acids.
    Methods Biochem Anal. 1966;14:113-76 PMID: 5328501
  55. Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.
    Proc Natl Acad Sci U S A. 1973 Apr;70(4):1218-21 PMID: 4515620
  56. Mechanism of hormonal induction of tyrosine aminotransferase studied by measurement of the concentration of growing enzyme molecules.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2937-41 PMID: 4404146
  57. Further studies on the stimulation of protein synthesis in androgen-dependent tissues by testosterone.
    Biochem J. 1972 Nov;130(1):189-97 PMID: 4655422
  58. Factors controlling amino acid incorporation by ribosomes from krebs II mouse ascites-tumour cells.
    Biochem J. 1966 Mar;98(3):826-35 PMID: 4287844
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1440413
Published
1974-11-00
Pages
413-26
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168510
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1440413 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2