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PMID: 4404146 Published · ppublish English Journal Article

Mechanism of hormonal induction of tyrosine aminotransferase studied by measurement of the concentration of growing enzyme molecules.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 10 ·1972-10-00 ·Pages 2937-41

Scott WA, Shields R, Tomkins GM

Abstract

Proteins labeled in cultured hepatoma cells grown in the presence of radioactive amino acids and pactamycin consist primarily of growing polypeptide chains initiated before addition of the antibiotic. Nascent tyrosine aminotransferase (EC 2.6.1.5) molecules were labeled in this way, and their radioactivity after completion was measured immunologically. Cells exposed to the synthetic adrenal steroid, dexamethasone, contain 10- to 18- times as much nascent enzyme as uninduced cells. These results indicate that induction control is not at the level of polypeptide-chain elongation or release, but probably operates on either the amount of messenger RNA or the rate of specific polypeptide-chain initiation.

MeSH Terms
Amino Acids/metabolism Animals Antibiotics, Antineoplastic/pharmacology Antibody Specificity Carbon Isotopes Carcinoma, Hepatocellular/enzymology,metabolism Cells, Cultured Centrifugation, Density Gradient Dexamethasone/pharmacology Electrophoresis, Polyacrylamide Gel Enzyme Induction/drug effects Immune Sera Liver Neoplasms Neoplasm Proteins/biosynthesis Peptide Chain Initiation, Translational/drug effects RNA, Messenger/biosynthesis RNA, Neoplasm/biosynthesis Rats Sheep/immunology Tritium Tyrosine Transaminase/biosynthesis
Chemicals
Amino Acids Antibiotics, Antineoplastic Carbon Isotopes Immune Sera Neoplasm Proteins RNA, Messenger RNA, Neoplasm Tritium Dexamethasone Tyrosine Transaminase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Scott W A
Shields R
Tomkins G M
References (13)
13 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.10.2937
Published
1972-10-00
Pages
2937-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389678
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.10.2937 PMC Full Text View journal details
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