Synthesis of inducible tyrosine aminotransferase in a cell-free extract from cultured hepatoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 12 ·1972-12-00 ·Pages 3615-9

Beck JP, Beck G, Wong KY, Tomkins GM

Abstract

A cell-free extract from cultured rat hepatoma cells is described that incorporates amino acids into chains of tyrosine aminotransferase that are identical, by several criteria, with tyrosine aminotransferase from rat liver. Extracts from steroid-induced cells are 6-to 10-times more active for tyrosine aminotransferase synthesis than extracts from uninduced cells, although the total incorporation rates are identical.

MeSH Terms

Amino Acids/metabolism Animals Carcinoma, Hepatocellular/enzymology Cell Fractionation Cell Line Cell-Free System Chromatography, DEAE-Cellulose Dexamethasone/pharmacology Electrophoresis, Polyacrylamide Gel Enzyme Induction/drug effects Liver Neoplasms Molecular Weight Neoplasms, Experimental/enzymology Polyribosomes/metabolism Precipitin Tests Radioimmunoassay Rats Tritium Tyrosine Transaminase/analysis,biosynthesis,isolation & purification

Chemicals

Amino Acids Tritium Dexamethasone Tyrosine Transaminase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Beck J P

Beck G

Wong K Y

Tomkins G M

References (13)

13 references, click to expand

Studies on the degradation of tyrosine aminotransferase in hepatoma cells in culture. Influence of the composition of the medium and adenosine triphosphate dependence.
J Biol Chem. 1971 Feb 10;246(3):710-4 PMID: 5542684
Role of DNA and specific cytoplasmic receptors in glucocorticoid action.
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1892-6 PMID: 4403081
Glucocorticoid receptors: relations between steroid binding and biological effects.
J Mol Biol. 1972 Jun 14;67(1):99-115 PMID: 4402888
Purification and properties of rat liver tyrosine aminotransferase.
J Biol Chem. 1969 Jul 10;244(13):3618-24 PMID: 4389463
Specific enzyme production in eukaryotic cells.
Adv Cell Biol. 1971;2:299-322 PMID: 4405117
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
A histochemical method for the demonstration of tyrosine aminotransferase in tissue culture cells, and studies of this enzyme in hepatoma tissue culture cells.
J Cell Biol. 1971 Jun;49(3):921-7 PMID: 4103958
Induction of tyrosine alpha-ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line.
Proc Natl Acad Sci U S A. 1966 Jul;56(1):296-303 PMID: 4381784
On the synthesis of tyrosine transaminase in vitro.
Biochim Biophys Acta. 1969 Feb 18;174(2):779-81 PMID: 4388602
Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell culture.
J Mol Biol. 1968 Jul 28;35(2):291-301 PMID: 4398833
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Partial purification by affinity chromatography of tyrosine aminotransferase-synthesizing ribosomes from hepatoma tissue culture cells.
Proc Natl Acad Sci U S A. 1971 May;68(5):1014-8 PMID: 4396918
Adaptation of the rat liver tyrosine-alpha-ketoglutarate transaminase.
Biochim Biophys Acta. 1957 Oct;26(1):85-8 PMID: 13479464

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.12.3615

Published

1972-12-00

Pages

3615-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389833

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.12.3615 PMC Full Text View journal details

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Synthesis of inducible tyrosine aminotransferase in a cell-free extract from cultured hepatoma cells.

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