Partial purification by affinity chromatography of tyrosine aminotransferase-synthesizing ribosomes from hepatoma tissue culture cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 5 ·1971-05-00 ·Pages 1014-8

Miller JV, Cuatrecasas P, Thompson EB

Abstract

This report describes a cell-free protein-synthesizing system derived from hepatoma tissue culture cells in which the synthesis of tyrosine aminotransferase is enhanced in relation to total protein synthesis. The enhancement was achieved by using affinity chromatography to select a specific fraction of ribosomes in which the proportion of tyrosine aminotransferase synthesis is higher than it is in unfractionated ribosomes.

MeSH Terms

Adsorption Animals Carbon Isotopes Carcinoma, Hepatocellular/enzymology Cell-Free System Chromatography Culture Techniques Immunoassay Liver Neoplasms Neoplasm Proteins/biosynthesis Picolines Polysaccharides Precipitin Tests Ribosomes/enzymology Tritium Tyrosine Transaminase/biosynthesis

Chemicals

Carbon Isotopes Neoplasm Proteins Picolines Polysaccharides Tritium Tyrosine Transaminase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Miller J V

Cuatrecasas P

Thompson E B

References (22)

22 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.5.1014

Published

1971-05-00

Pages

1014-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389103

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.5.1014 PMC Full Text View journal details

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Partial purification by affinity chromatography of tyrosine aminotransferase-synthesizing ribosomes from hepatoma tissue culture cells.

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