Differences between the integration of avian myeloblastosis virus DNA in leukemic cells and of endogenous viral DNA in normal chicken cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3152-6

Evans RM, Baluda MA, Shoyab M

Abstract

The nature of integrated viral DNA in normal and leukemic chicken cells has been studied by sequential nucleic acid hybridization procedures that localize the viral specific DNA in cellular DNA regions differing in reiteration frequency. First, DNA.DNA reassociation was employed to fractionate cellular DNA sequences according to their reiteration frequencies. Next, the DNA in each fraction was denatured, immobilized on nitrocellulose filters, and then hybridized with viral [(3)H]RNA. In normal cells, endogenous viral DNA appears to be associated with cell sequences reiterated 1200 times, and each integration unit appears to have a maximal size approximately equivalent to the 35S RNA subunit of the virion. In infected cells, additional viral sequences are found which reassociate as if they integrated adjacent to unique cellular DNA, or in tandem with endogenous viral DNA.

MeSH Terms

Animals Avian Leukosis Virus/metabolism Avian Myeloblastosis Virus/metabolism Chick Embryo Chickens DNA, Neoplasm/metabolism DNA, Single-Stranded/metabolism DNA, Viral/metabolism Kinetics Leukemia, Myeloid, Acute/metabolism Lysogeny Nucleic Acid Hybridization Nucleic Acid Renaturation RNA, Viral/metabolism Recombination, Genetic

Chemicals

DNA, Neoplasm DNA, Single-Stranded DNA, Viral RNA, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Evans R M

Baluda M A

Shoyab M

References (24)

24 references, click to expand

Integrated state of oncornavirus DNA in normal chicken cells and in cells transformed by avian myeloblastosis virus.
J Virol. 1973 Oct;12(4):721-32 PMID: 4359950
Nuclear DNA sequences present in human leukemic cells and absent in normal leukocytes.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3737-41 PMID: 4509337
Identification of a developmental program using bromodeoxyuridine.
J Mol Biol. 1972 Sep 28;70(2):337-50 PMID: 5078575
Measurement of endogenous leukosis virus nucleotide sequences in the DNA of normal avian embryos by RNA-DNA hybridization.
Virology. 1973 May;53(1):196-203 PMID: 4350417
General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus.
J Mol Biol. 1973 Jun 15;77(1):1-23 PMID: 4769838
Kinetics of renaturation of DNA.
J Mol Biol. 1968 Feb 14;31(3):349-70 PMID: 5637197
The integrated state of viral DNA in SV40-transformed cells.
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288-95 PMID: 4299943
A DNA fragment of Herpes simplex 2 and its transcription in human cervical cancer tissue.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3784-9 PMID: 4345508
Characterization of murine leukaemia virus-specific DNA present in normal mouse cells.
Nat New Biol. 1973 Jul 18;244(133):76-9 PMID: 4352888
Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.
Science. 1968 Aug 9;161(3841):529-40 PMID: 4874239
Induction of avian tumor viruses in normal cells by physical and chemical carcinogens.
Virology. 1971 Dec;46(3):920-38 PMID: 4332981
Three size-classes of intracellular adenovirus deoxyribonucleic acid.
J Virol. 1971 Jun;7(6):707-19 PMID: 5105670
Integration of simian virus 40 deoxyribonucleic acid into the deoxyribonucleic acid of permissive monkey kidney cells.
J Virol. 1972 Apr;9(4):705-7 PMID: 4336565
Prophage P1, and extrachromosomal replication unit.
Cold Spring Harb Symp Quant Biol. 1968;33:791-8 PMID: 4892009
Simian virus 40 DNA integration within the genome of virus-transformed mammalian cells.
Virology. 1973 Feb;51(2):351-7 PMID: 4348303
'Integration' of polyoma virus DNA into mammalian genomes.
Biochem Biophys Res Commun. 1972 Apr 14;47(1):111-8 PMID: 4337421
The RNA tumor viruses--background and foreground.
Proc Natl Acad Sci U S A. 1972 Apr;69(4):1016-20 PMID: 4337233
Rous sarcoma virus nucleotide sequences in cellular DNA: measurement by RNA-DNA hybridization.
Science. 1972 Nov 17;178(4062):750-3 PMID: 4343259
Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses.
Proc Natl Acad Sci U S A. 1972 Mar;69(3):576-80 PMID: 4335067
Synthesis of avian oncornavirus DNA in infected chicken cells.
J Virol. 1974 May;13(5):1005-13 PMID: 4363246
Acquisition of new DNA sequences after infection of chicken cells with avian myeloblastosis virus.
J Virol. 1974 Feb;13(2):331-9 PMID: 16789139
Presence in leukemic cells of avian myeloblastosis virus-specific DNA sequences absent in normal chicken cells.
J Virol. 1974 Jul;14(1):47-9 PMID: 4365719
Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts.
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3067-71 PMID: 4361674
Lysogeny: the integration problem.
Annu Rev Microbiol. 1968;22:451-88 PMID: 4879520

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.8.3152

Published

1974-08-00

Pages

3152-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388640

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.8.3152 PMC Full Text View journal details

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Differences between the integration of avian myeloblastosis virus DNA in leukemic cells and of endogenous viral DNA in normal chicken cells.

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