A DNA fragment of Herpes simplex 2 and its transcription in human cervical cancer tissue.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 12 ·1972-12-00 ·Pages 3784-9

Frenkel N, Roizman B, Cassai E, Nahmias A

Abstract

A human cervical tumor, free of detectable infectious herpes simplex 2 virus, contained a fragment comprising 39% of herpes viral DNA. Renaturation kinetics indicate that an average of 1 to 3.5 DNA fragments of herpes simplex virus are present per cell, depending on the ploidy of the cells in this particular tumor. Virus-specific sequences were found linked to highly repetitive sequences of host DNA, which reassociated under conditions designed to preclude reassociation of viral sequences. The tumor also contained RNA transcripts complementary to 5% of the viral DNA. The fraction of viral DNA template transcribed in the cervical tumor is considerably less than that transcribed in productively infected cells (50%).

MeSH Terms

DNA, Neoplasm/analysis DNA, Viral/analysis,isolation & purification,metabolism Female Humans Kinetics Middle Aged Nucleic Acid Hybridization Nucleic Acid Renaturation RNA, Neoplasm/metabolism RNA, Viral/analysis Simplexvirus/isolation & purification Templates, Genetic Transcription, Genetic Uterine Cervical Neoplasms/metabolism,microbiology

Chemicals

DNA, Neoplasm DNA, Viral RNA, Neoplasm RNA, Viral

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Frenkel N

Roizman B

Cassai E

Nahmias A

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.12.3784

Published

1972-12-00

Pages

3784-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389873

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.12.3784 PMC Full Text View journal details

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A DNA fragment of Herpes simplex 2 and its transcription in human cervical cancer tissue.

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