Nuclear ribonucleoprotein complexes containing polyadenylate from mouse ascites cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 7 ·1974-07-00 ·Pages 2632-6

Quinlan TJ, Billings PB, Martin TE

Abstract

Nuclear poly(A)-containing RNA of mouse ascites cells can be extracted in the form of 15-17S ribonucleoprotein complexes under conditions in which the bulk of the heterogeneous nuclear RNA is released as 30S complexes. The poly(A)-containing fraction of nuclear extracts has been resolved into two distinct components, 15 and 17 S; neither contains the two polypeptides of 30S ribonucleoprotein. The 17S particle contains approximately six polypeptide species of molecular masses 17,000-30,000 daltons. The 15S complex has four distinct polypeptides of higher molecular mass, including a prominent 80,000-dalton species.

MeSH Terms

Adenine Nucleotides/analysis Adenosine/metabolism Animals Ascitic Fluid/cytology Carcinoma, Hepatocellular/metabolism Cell Line Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Liver Neoplasms/metabolism Mice Micropore Filters Neoplasms, Experimental/metabolism Nucleoproteins/analysis Peptides/analysis Polynucleotides/analysis RNA/analysis Tritium

Chemicals

Adenine Nucleotides Nucleoproteins Peptides Polynucleotides Tritium RNA Adenosine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Quinlan T J

Billings P B

Martin T E

References (31)

31 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.7.2632

Published

1974-07-00

Pages

2632-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388520

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.7.2632 PMC Full Text View journal details

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Nuclear ribonucleoprotein complexes containing polyadenylate from mouse ascites cells.

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