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PMID: 4508317 Published · ppublish English Journal Article

A particle associated with the polyadenylate segment in mammalian messenger RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 11 ·1972-11-00 ·Pages 3247-50

Kwan SW, Brawerman G

Abstract

A structure consisting of poly(A) complexed with other components is released from polysomes by ribonuclease treatment. The poly(A) complex has a sedimentation value of 12-15, while the corresponding sedimentation value for free poly(A) is 4. The complex does not appear to represent an artifact formed by interaction of free poly(A) with either cytoplasmic or polysomal proteins. The polynucleotide released from the complex by treatment with sodium dodecyl sulfate shows the same electrophoretic mobility as that of poly(A) isolated from deproteinized polysomal RNA. The poly(A) in the complex is partially protected from digestion by T(2) ribonuclease. At least part of the poly(A) is available for base pairing with poly(U). The components associated with the poly(A) cause it to bind to Millipore filters at low ionic strength. These components are removed from the complex by Pronase digestion. The findings indicate that the poly(A) segment in messenger RNA serves as a binding site for a particle. This particle appears to consist of proteins.

MeSH Terms
Animals Binding Sites Carbon Isotopes Cells, Cultured Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Mice Micropore Filters Models, Structural Nucleic Acid Hybridization Poly U Polynucleotides/analysis,metabolism Polyribosomes/analysis,metabolism Pronase/metabolism Protein Binding RNA, Messenger/analysis,metabolism RNA, Neoplasm/analysis RNA, Ribosomal/analysis,metabolism Ribonucleases/metabolism Sarcoma 180 Sodium Dodecyl Sulfate Tritium
Chemicals
Carbon Isotopes Polynucleotides RNA, Messenger RNA, Neoplasm RNA, Ribosomal Tritium Poly U Sodium Dodecyl Sulfate Ribonucleases Pronase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kwan S W
Brawerman G
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.11.3247
Published
1972-11-00
Pages
3247-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389746
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.11.3247 PMC Full Text View journal details
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