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PMID: 4350707 Published · ppublish English Journal Article

Molecular weight determination of Sendai RNA by dimethyl sulfoxide gradient sedimentation.

Journal of virology ·Vol. 11 ·No. 5 ·1973-05-00 ·Pages 615-20

Kolakofsky D, Bruschi A

Abstract

The molecular weight of the large RNA of Sendai virus has been determined by sedimentation analysis in sucrose gradients containing 99% dimethyl sulfoxide (DMSO) to be 2.3 x 10(6). Sendai RNA recovered from 99% DMSO was found to cosediment with nondenatured Sendai RNA at 46 to 48s in ordinary sucrose gradients. The molecular weight value of 2.3 x 10(6) is considerably smaller than the estimates of 6 x 10(6) to 7 x 10(6) determined under nondenaturing conditions, suggesting a unique structure for Sendai RNA.

MeSH Terms
Animals Carbon Isotopes Cells, Cultured/analysis Centrifugation, Density Gradient Chick Embryo Dimethyl Sulfoxide Kidney Methods Mice Molecular Weight Newcastle disease virus/analysis,growth & development Nucleic Acid Denaturation Parainfluenza Virus 1, Human/analysis,growth & development Phosphorus Isotopes RNA/analysis RNA, Ribosomal/analysis RNA, Viral/analysis,isolation & purification Sucrose Tritium Uridine Virus Cultivation
Chemicals
Carbon Isotopes Phosphorus Isotopes RNA, Ribosomal RNA, Viral Tritium Sucrose RNA Uridine Dimethyl Sulfoxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kolakofsky D
Bruschi A
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26 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.11.5.615-620.1973
Published
1973-05-00
Pages
615-20
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355155
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.11.5.615-620.1973 PMC Full Text View journal details
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