Isolation and properties of Newcastle disease virus nucleocapsid.

Journal of virology ·Vol. 2 ·No. 3 ·1968-03-00 ·Pages 248-55

Kingsbury DW, Darlington RW

Abstract

Deoxycholate (DOC) disrupted virions of Newcastle disease virus (NDV), releasing viral nucleocapsids. The nucleocapsids sedimented at about 200S in sucrose gradients and measured from 1.3 to 1.4 mu long by electron microscopy. NDV nucleo-capsids were resistant to pancreatic ribonuclease. These nucleocapsids contained all the 50S ribonucleic acid (RNA) in NDV virions, while virus-associated RNA sedimenting at less than 50S was external to the virions.

MeSH Terms

Bile Acids and Salts/pharmacology Centrifugation, Density Gradient Cholanes/pharmacology Microscopy, Electron Newcastle disease virus/analysis Nucleoproteins/isolation & purification RNA, Viral/isolation & purification Ribonucleases/pharmacology Tritium Viral Proteins/isolation & purification

Chemicals

Bile Acids and Salts Cholanes Nucleoproteins RNA, Viral Viral Proteins Tritium Ribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kingsbury D W

Darlington R W

References (12)

12 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.2.3.248-255.1968

Published

1968-03-00

Pages

248-55

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC375607

Subset

External Links

PubMed source DOI: 10.1128/JVI.2.3.248-255.1968 PMC Full Text View journal details

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Isolation and properties of Newcastle disease virus nucleocapsid.

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