Initiation and direction of RNA transcription by vesicular stomatitis virus virion transcriptase.

Journal of virology ·Vol. 11 ·No. 4 ·1973-04-00 ·Pages 487-501

Roy P, Bishop DH

Abstract

The initiation of RNA transcription by the virion-bound RNA transcriptase of vesicular stomatitis virus has been examined. Multiple initiation sequences have been observed, two of which have been characterized (pppApCpGp... and pppGpCp...) suggestive of a transcription process which can start at different sites along the template RNA. By the use of sequential labeling techniques and exonucleases, it has been determined that there is a 5' to 3' direction of product RNA synthesis.

MeSH Terms

Base Sequence Chromatography Chromatography, Thin Layer DNA-Directed RNA Polymerases/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Hydroxides Nucleoproteins/metabolism Nucleotides/metabolism Phosphorus Isotopes RNA, Viral/analysis,biosynthesis Ribonucleases Transcription, Genetic Tritium Vesicular stomatitis Indiana virus/enzymology,metabolism Viral Proteins/metabolism

Chemicals

Hydroxides Nucleoproteins Nucleotides Phosphorus Isotopes RNA, Viral Viral Proteins Tritium DNA-Directed RNA Polymerases Ribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Roy P

Bishop D H

References (18)

18 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.11.4.487-501.1973

Published

1973-04-00

Pages

487-501

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355129

Subset

External Links

PubMed source DOI: 10.1128/JVI.11.4.487-501.1973 PMC Full Text View journal details

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Initiation and direction of RNA transcription by vesicular stomatitis virus virion transcriptase.

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