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PMID: 4342241 Published · ppublish English Journal Article

Dissociation of vesicular stomatitis virus and relation of the virion proteins to the viral transcriptase.

Journal of virology ·Vol. 10 ·No. 2 ·1972-08-00 ·Pages 234-43

Bishop DH, Roy P

Abstract

A procedure has been developed for the sequential removal and purification of the glycoprotein and membrane protein of vesicular stomatitis virus (VSV). Neither of these proteins exhibited transcriptase activity. All of the activity was recovered in the ribonucleic acid (RNA)-ribonucleoprotein complex of VSV, which also has four other minor proteins associated with it. During transcription of 41% of the RNA of a virus preparation, no dissociation of the ribonucleoprotein from the viral RNA was observed.

MeSH Terms
Chromatography, Gel DNA-Directed RNA Polymerases/metabolism Dextrans Electrophoresis, Polyacrylamide Gel Genetics, Microbial Glycols Glycoproteins/isolation & purification Phosphorus Isotopes Polysaccharides RNA, Viral/analysis,biosynthesis,isolation & purification Transcription, Genetic Tritium Uracil Nucleotides/metabolism Vesicular stomatitis Indiana virus/analysis,enzymology,metabolism Viral Proteins/isolation & purification
Chemicals
Dextrans Glycols Glycoproteins Phosphorus Isotopes Polysaccharides RNA, Viral Uracil Nucleotides Viral Proteins Tritium DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bishop D H
Roy P
References (23)
23 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.2.234-243.1972
Published
1972-08-00
Pages
234-43
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356455
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.2.234-243.1972 PMC Full Text View journal details
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