Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus.

Journal of virology ·Vol. 7 ·No. 6 ·1971-06-00 ·Pages 726-35

Aaslestad HG, Clark HF, Bishop DH, Koprowski H

Abstract

A ribonucleic acid (RNA)-dependent RNA polymerase has been demonstrated in Kern Canyon virus (KCV) particles. The RNA product of the KCV polymerase hybridizes to KCV viral RNA. The properties of this viral enzyme have been characterized and compared with those of vesicular stomatitis virus (VSV). RNA polymerases from both viruses require similar conditions of temperature, pH, and detergent and magnesium concentrations for maximal synthesis of RNA. The RNA polymerase contained in the virion of KCV was more dependent on the presence of a sulfhydryl agent than was the VSV enzyme. Under optimal conditions, the specific activity of the VSV polymerase is about twenty-five times as great as that of KCV.

MeSH Terms

Animals Carbon Isotopes Cell Line Centrifugation, Density Gradient Chemical Precipitation Cricetinae Culture Media Guanine Nucleotides/metabolism Hydrogen-Ion Concentration Kidney Magnesium/pharmacology Nucleic Acid Hybridization Quaternary Ammonium Compounds RNA Nucleotidyltransferases/isolation & purification,metabolism RNA, Viral/biosynthesis Species Specificity Sucrose Sulfates Sulfhydryl Reagents/pharmacology Temperature Tritium Uracil Nucleotides/metabolism Uridine/metabolism Vesicular stomatitis Indiana virus/enzymology,isolation & purification,metabolism,pathogenicity Viruses, Unclassified/enzymology,isolation & purification,metabolism,pathogenicity

Chemicals

Carbon Isotopes Culture Media Guanine Nucleotides Quaternary Ammonium Compounds RNA, Viral Sulfates Sulfhydryl Reagents Uracil Nucleotides Tritium Sucrose RNA Nucleotidyltransferases Magnesium Uridine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Aaslestad H G

Clark H F

Bishop D H

Koprowski H

References (14)

14 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.7.6.726-735.1971

Published

1971-06-00

Pages

726-35

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356191

Subset

External Links

PubMed source DOI: 10.1128/JVI.7.6.726-735.1971 PMC Full Text View journal details

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Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus.

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