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PMID: 4987176 Published · ppublish English Journal Article

Reproducible plaquing system for rabies, lymphocytic choriomeningitis,k and other ribonucleic acid viruses in BHK-21-13S agarose suspensions.

Journal of virology ·Vol. 1 ·No. 6 ·1967-12-00 ·Pages 1224-6

Sedwick WD, Wiktor TJ

Abstract

BHK21/13S-agarose suspension cultures provide an excellent plaquing system for rabies and lymphocytic choriomeningitis viruses. The system has facilitated accurate and reproducible assay of serum neutralization and cloning of these viruses. Rubella, reo, and Kern Canyon viruses may also be assayed by this technique.

MeSH Terms
Animals Antibody Formation Cattle Cell Line Clone Cells Cricetinae Culture Media Culture Techniques Hemolytic Plaque Technique Kidney Lymphocytic Choriomeningitis/microbiology Lymphocytic choriomeningitis virus/growth & development Methods Rabbits Rabies virus/growth & development,immunology Virus Cultivation Viruses, Unclassified/growth & development
Chemicals
Culture Media
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sedwick W D
Wiktor T J
References (8)
8 references, click to expand
  1. Cytopathic effect of rubella virus in RHK21 cells and growth to high titers in suspension culture.
    Virology. 1965 Oct;27(2):239-41 PMID: 5320494
  2. Rabies and lymphocytic choriomeningitis virus (LCMV) infection of tissue culture; enhancing effect of LCMV.
    Ann Med Exp Biol Fenn. 1966;44(2):290-6 PMID: 4961010
  3. Two spontaneously transformed cell lines derived from the same hamster embryo culture.
    Int J Cancer. 1967 Mar 15;2(2):143-52 PMID: 6039764
  4. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence.
    Virology. 1962 Feb;16:147-51 PMID: 14468055
  5. In vitro differentiation of virulent and attenuated polioviruses by their growth characteristics on MS cells.
    J Exp Med. 1959 Jan 1;109(1):9-24 PMID: 13611161
  6. MECHANISM OF THE CYTOPATHIC EFFECT OF RABIES VIRUS IN TISSUE CULTURE.
    Virology. 1963 Sep;21:128-31 PMID: 14062905
  7. CULTIVATION OF RABIES VIRUS IN HUMAN DIPLOID CELL STRAIN WI-38.
    J Immunol. 1964 Sep;93:353-66 PMID: 14218592
  8. New assay procedure for separation of mycoplasmas from virus pools and tissue culture systems.
    J Virol. 1967 Dec;1(6):1227-37 PMID: 4912246
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.1.6.1224-1226.1967
Published
1967-12-00
Pages
1224-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC375413
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.1.6.1224-1226.1967 PMC Full Text View journal details
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