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PMID: 4328752 Published · ppublish English Journal Article

Asymmetric template function of microbial deoxyribonucleic acids: transcription of messenger ribonucleic acid.

Journal of bacteriology ·Vol. 107 ·No. 3 ·1971-09-00 ·Pages 610-7

Margulies L, Remeza V, Rudner R

Abstract

In Bacillus subtilis and Escherichia coli, pulse-labeled ribonucleic acid (RNA) synthesized during step-down growth hybridized preferentially with the heavy (H) strand of methylated albumin-Kieselguhr-fractionated deoxyribonucleic acid (DNA). At high RNA inputs, the ratio of RNA hybridized with the H strand to that hybridized with the light (L) strand was 8.7 for B. subtilis and 2.0 for E. coli. At high DNA inputs, the H/L hybridization ratio increased by a factor of two. This change in the hybridization ratio was attributable to the fraction of the pulse-labeled RNA which is in stable RNA components. The hybridization peak of pulse-labeled RNA was specifically located in the late-eluting region of the absorbance profile of the H strand. This region was considered to represent the most actively transcribing H strand templates.

MeSH Terms
Bacillus subtilis/metabolism Chromatography DNA, Bacterial/isolation & purification Escherichia coli/metabolism Genetic Code Genetics, Microbial Nucleic Acid Denaturation Nucleic Acid Hybridization RNA, Bacterial/biosynthesis RNA, Messenger RNA, Ribosomal Serum Albumin, Bovine Silicon Dioxide Templates, Genetic Tritium Uridine/metabolism
Chemicals
DNA, Bacterial RNA, Bacterial RNA, Messenger RNA, Ribosomal Tritium Serum Albumin, Bovine Silicon Dioxide Uridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Margulies L
Remeza V
Rudner R
References (29)
29 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.107.3.610-617.1971
Published
1971-09-00
Pages
610-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246978
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.107.3.610-617.1971 PMC Full Text View journal details
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