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PMID: 4980029 Published · ppublish English Journal Article

Separation of microbial deoxyribonucleic acids into complementary strands.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 1 ·1969-05-00 ·Pages 152-9

Rudner R, Karkas JD, Chargaff E

Abstract

DNA preparations from seven bacterial species and from the E. coli phage T4 can, after denaturation with alkali, be separated chromatographically into two distinct components (L and H) through intermittent gradient elution from methylated albumin kieselguhr columns. The direct chemical analysis of the L and H fractions isolated from DNA specimens of the AT type shows them to exhibit a high degree of complementarity; but despite a bias in the distribution of purines and pyrimidines, either fraction contains equimolar quantities of 6-amino and of 6-keto nucleotides. In the L and H components derived from DNA of the equimolar and GC types, the distribution bias appears limited to guanine and cytosine. It is suggested that the L and H fractions represent the complementary DNA strands.

MeSH Terms
Bacillus Bacillus megaterium Bacillus subtilis Chromatography Coliphages DNA, Bacterial/analysis,isolation & purification DNA, Viral/isolation & purification Escherichia coli Methods Nucleic Acid Denaturation Nucleotides/analysis Proteus Salmonella typhimurium Serratia marcescens
Chemicals
DNA, Bacterial DNA, Viral Nucleotides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rudner R
Karkas J D
Chargaff E
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.63.1.152
Published
1969-05-00
Pages
152-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC534015
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.63.1.152 PMC Full Text View journal details
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