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PMID: 4319834 Published · ppublish English Journal Article

Asymmetric template function of microbial deoxyribonucleic acids: transcription of ribosomal and soluble ribonucleic acids.

Journal of bacteriology ·Vol. 103 ·No. 3 ·1970-09-00 ·Pages 560-8

Margulies L, Remeza V, Rudner R

Abstract

In Bacillus subtilis and Escherichia coli, 16 and 23S ribosomal ribonucleic acid (rRNA) hybridize exclusively with the heavy (H) strand of methylated albuminkieselguhr (MAK)-fractionated complementary deoxyribonucleic acid (DNA) strands. All the soluble RNA (4S RNA) in B. subtilis and 66 to 75% of the 4S RNA in E. coli also hybridize with the H strand. Interspecific hybridization shows that E. coli 23S rRNA also binds selectively to the DNA H strand of Salmonella typhimurium. The hybridization peak for all three cellular RNA components is specifically located in the late-eluting region of the absorbance profile of the DNA H strand. The early-eluting region of the light (L) strand preferentially inhibits the hybridization between the peak region of the H strand and 23S rRNA. These regions are considered to represent the transcribing sequences and their complements for 23S rRNA in the separated H and L strands of DNA, respectively.

MeSH Terms
Albumins Bacillus subtilis Chromatography DNA, Bacterial/isolation & purification Escherichia coli Genetic Code Genetics, Microbial Hybridization, Genetic RNA, Bacterial/isolation & purification Ribosomes Salmonella typhimurium Silicon Dioxide Templates, Genetic Tritium Uracil
Chemicals
Albumins DNA, Bacterial RNA, Bacterial Tritium Uracil Silicon Dioxide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Margulies L
Remeza V
Rudner R
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40 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.103.3.560-568.1970
Published
1970-09-00
Pages
560-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248127
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.103.3.560-568.1970 PMC Full Text View journal details
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