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PMID: 4209774 Published · ppublish English Journal Article

Thymineless death in Bacillus subtilis: correlation between cell lysis and deoxyribonucleic acid breakdown.

Journal of bacteriology ·Vol. 119 ·No. 1 ·1974-07-00 ·Pages 36-43

Ephrati-Elizur E, Yosuv D, Shmueli E, Horowitz A

Abstract

Bacillus subtilis carrying an inducible defective phage is several times more sensitive to thymineless death than a mutagenized derivative that behaves as a nonlysogen. When the integrity of the deoxyribonucleic acid (DNA) of both strains was examined during thymine starvation by transformation experiments, sedimentation studies, and measurements of acid-soluble DNA degradation products, it was shown that extensive DNA breakdown occurred only in the lysogenic strain. During thymine starvation of this strain, there is a progressive proclivity to lysis, followed by leakage of DNA and DNA degradation products. Such leakage was not observed in the nonlysogen. A correlation between proclivity to lysis and extensive DNA degradation is indicated.

MeSH Terms
Adenine/metabolism Bacillus subtilis/metabolism Carbon Radioisotopes Centrifugation, Density Gradient DNA, Bacterial/biosynthesis,metabolism Lysogeny Mitomycins/pharmacology Mutagens Mutation Nitrosoguanidines Nucleic Acid Denaturation Rifampin/pharmacology Thymidine/metabolism Thymine/metabolism Transformation, Genetic Tritium
Chemicals
Carbon Radioisotopes DNA, Bacterial Mitomycins Mutagens Nitrosoguanidines Tritium Adenine Thymine Thymidine Rifampin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ephrati-Elizur E
Yosuv D
Shmueli E
Horowitz A
References (39)
39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.119.1.36-43.1974
Published
1974-07-00
Pages
36-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245569
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.119.1.36-43.1974 PMC Full Text View journal details
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