Alkaline sucrose gradient sedimentation of chromosomal deoxyribonucleic acid from Escherichia coli PolA + and PolA - strains during thymine starvation.

Journal of bacteriology ·Vol. 108 ·No. 3 ·1971-12-00 ·Pages 1422-3

Sedgwick SG, Bridges BA

Abstract

Single-strand breaks were not detected in the deoxyribonucleic acid of Escherichia coli after thymine starvation for up to 180 min, even in a sensitive PolA(-) strain.

MeSH Terms

Bacteriological Techniques Centrifugation, Density Gradient Chromosomes, Bacterial/analysis Culture Media DNA Nucleotidyltransferases/metabolism DNA Repair DNA, Bacterial/analysis Escherichia coli/analysis,enzymology,growth & development,metabolism Mutation Sucrose Thymidine/metabolism Thymine/metabolism Transduction, Genetic Tritium

Chemicals

Culture Media DNA, Bacterial Tritium Sucrose DNA Nucleotidyltransferases Thymine Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sedgwick S G

Bridges B A

References (9)

9 references, click to expand

Thymine Starvation and Single-Strand Breaks in Chromosomal Deoxyribonucleic acid of Escherichia coli.
J Bacteriol. 1970 Dec;104(3):1391-2 PMID: 16559119
Influence of thymine starvation on the integrity of deoxyribonucleic acid in Escherichia coli.
J Bacteriol. 1971 Mar;105(3):733-8 PMID: 4926681
Molecular mechanism of thymine-less death.
Biochem Biophys Res Commun. 1962 Nov 27;9:398-404 PMID: 13934873
DNA polymerase required for rapid repair of x-ray--induced DNA strand breaks in vivo.
Science. 1971 May 21;172(3985):851-4 PMID: 4929534
Isolation of an E. coli strain with a mutation affecting DNA polymerase.
Nature. 1969 Dec 20;224(5225):1164-6 PMID: 4902142
The effect of thymine starvation on chromosomal structure of Escherichia coli JG-151.
Biochem Biophys Res Commun. 1971 Jan 8;42(1):23-30 PMID: 4927142
Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces.
Nature. 1966 Oct 29;212(5061):534-5 PMID: 5339143
STUDIES ON UNBALANCED GROWTH IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1954 Oct;40(10):885-93 PMID: 16589586
Genetic and molecular characteristics of X-ray-sensitive mutants of Escherichia coli defective in repair synthesis.
J Bacteriol. 1970 Nov;104(2):871-81 PMID: 4923076

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.108.3.1422-1423.1971

Published

1971-12-00

Pages

1422-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247235

Subset

External Links

PubMed source DOI: 10.1128/jb.108.3.1422-1423.1971 PMC Full Text View journal details

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Alkaline sucrose gradient sedimentation of chromosomal deoxyribonucleic acid from Escherichia coli PolA + and PolA - strains during thymine starvation.

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