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PMID: 4926681 Published · ppublish English Journal Article

Influence of thymine starvation on the integrity of deoxyribonucleic acid in Escherichia coli.

Journal of bacteriology ·Vol. 105 ·No. 3 ·1971-03-00 ·Pages 733-8

Baker ML, Hewitt RR

Abstract

The influence of thymine starvation on the single-strand molecular weight of deoxyribonucleic acid (DNA) from Escherichia coli was determined by sedimentation through gradients of alkaline sucrose. Growth of cells for as long as 150 min in thymineless medium did not significantly reduce the molecular weight below the control value of 2.4 +/- 0.3 x 10(8) daltons. Incubation of cells in thymineless medium after exposure to 500 ergs/mm(2) of ultraviolet light or 20 krad of (137)Cs gamma rays did not appear to block the rejoining of single-strand breaks associated with irradiation. Thus, DNA repair enzymes, presumably including DNA ligase, are not significantly inhibited by thymine starvation.

MeSH Terms
Carbon Isotopes Centrifugation, Density Gradient Cesium Isotopes Culture Media DNA Replication DNA, Bacterial/radiation effects Escherichia coli/enzymology,growth & development,metabolism,radiation effects Genetics, Microbial Ligases/metabolism Molecular Weight Nucleic Acid Denaturation Sucrose Thymine/metabolism Tritium Ultraviolet Rays
Chemicals
Carbon Isotopes Cesium Isotopes Culture Media DNA, Bacterial Tritium Sucrose Ligases Thymine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baker M L
Hewitt R R
References (18)
18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.105.3.733-738.1971
Published
1971-03-00
Pages
733-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248494
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.105.3.733-738.1971 PMC Full Text View journal details
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