Utilization of 5-bromouracil by thymineless bacteria.

Journal of bacteriology ·Vol. 93 ·No. 1 ·1967-01-00 ·Pages 86-9

Hewitt R, Suit JC, Billen D

Abstract

Several thymineless Escherichia coli strains have been examined for their ability to replicate their deoxyribonucleic acid when bromouracil is substituted for thymine. The procedure we describe was used to identify a thymineless strain with characteristics relatively favorable to its use in bromouracil labeling experiments. In addition, mutants with an "absolute" thymine requirement could be easily distinguished from one with a "leaky" thymine requirement.

MeSH Terms

Bromine/metabolism Carbon Isotopes DNA, Bacterial/biosynthesis Escherichia coli/metabolism Mutation Thymine/metabolism Tritium Ultracentrifugation Uracil/metabolism

Chemicals

Carbon Isotopes DNA, Bacterial Tritium Uracil Thymine Bromine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hewitt R

Suit J C

Billen D

References (10)

10 references, click to expand

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Alterations in the radiosensitivity of Escherichia coli through modification of cellular macromolecular components.
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Thymine incorporation and metabolism by various classes of thymine-less bacteria.
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Regulation of chromosome replication and segregation in bacteria.
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J Mol Biol. 1964 Aug;9:395-410 PMID: 14202275
THE REPLICATION OF DNA IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671-82 PMID: 16590258
Lethal and Sublethal Effects of X-Rays on Escherichia coli as Related to the Yield of Biochemical Mutants.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.93.1.86-89.1967

Published

1967-01-00

Pages

86-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC314972

Subset

External Links

PubMed source DOI: 10.1128/jb.93.1.86-89.1967 PMC Full Text View journal details

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Utilization of 5-bromouracil by thymineless bacteria.

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