Home LiteratureArticle Details
PMID: 4208074 Published · ppublish English Journal Article

Action of cytochalasin D on cells of established lines. I. Early events.

The Journal of cell biology ·Vol. 61 ·No. 2 ·1974-05-00 ·Pages 481-500

Miranda AF, Godman GC, Deitch AD, Tanenbaum SW

Abstract

HeLa, Vero, L, HEp2, and MDBK cells respond immediately to 0.2-0.5 microg/ml cytochalasin D (CD) with sustained contraction (contracture), loss of microvilli, expression of endoplasmic contents (zeiosis), nuclear protrusion, and extension of cytoplasmic processes. The development of these changes is depicted, and the dose-response patterns in these cell lines are described. MDBK is generally most resistant and HeLa most sensitive to these effects of CD. Cells in G(1) are most sensitive to CD; responsiveness decreases progressively during early S and is least in mid S through G(2). CD inhibits transport of [(14)C]deoxyglucose in HeLa by about 45% but has no significant effect on hexose uptake in Vero and MDBK; sugar transport is thus apparently unrelated to any morphologic effect of CD. Although spreading and attachment are impeded, CD does not decrease and may even enhance the adhesiveness of established monolayers. Contraction appears to be a primary early effect of CD, upon which other visible changes follow. It is prevented by some inhibitors of energy metabolism (deoxyglucose and dinitrophenol) and does not occur in glycerinated models without ATP. The possible bases of the contractile response to CD are discussed. Although direct or indirect action of CD on some microfilaments may occur, a generalized structural disruption of contractile filaments by CD is considered unlikely.

MeSH Terms
Animals Autoradiography Carbon Radioisotopes Carcinoma, Squamous Cell Cattle Cell Adhesion/drug effects Cell Line/drug effects Cell Membrane Permeability/drug effects Cell Nucleus/drug effects Cytochalasins/pharmacology Cytoplasm/drug effects Glucose/metabolism Haplorhini HeLa Cells Humans Kidney L Cells Laryngeal Neoplasms Mice Microscopy, Electron Microscopy, Electron, Scanning Species Specificity Tritium
Chemicals
Carbon Radioisotopes Cytochalasins Tritium Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miranda A F
Godman G C
Deitch A D
Tanenbaum S W
References (85)
85 references, click to expand
  1. Cytokinesis and cytochalasin-induced furrow regression in the first-cleavage zygote of Xenopus laevis.
    Z Zellforsch Mikrosk Anat. 1971;121(1):102-26 PMID: 4329592
  2. A light-microscope study of the action of cytochalasin B on the cells and isolated cytoplasm of the characeae.
    J Cell Sci. 1972 May;10(3):811-9 PMID: 5038417
  3. The sensitivity of developing cardiac myofibrils to cytochalasin-B (electron microscopy-polarized light-Z-bands-heartbeat).
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):308-12 PMID: 4501115
  4. Isolation and characterization of myosin and two myosin fragments from human blood platelets.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2703-7 PMID: 4256552
  5. A contractile protein possessing Ca 2+ sensitivity (natural actomyosin) from leucocytes. Its extraction and some of its properties.
    Biochim Biophys Acta. 1972 Feb 28;256(2):565-76 PMID: 4259563
  6. Effect of cytochalasin B and colchicine on the stimulation of -amylase release from rat parotid tissue slices.
    Biochem Biophys Res Commun. 1972 Jul 11;48(1):23-9 PMID: 4339462
  7. The inhibition of sugar transport in chick embryo fibroblasts by cytochalasin B. Evidence for a membrane-specific effect.
    J Biol Chem. 1973 Jan 25;248(2):711-9 PMID: 4734334
  8. Factors controlling cytoplasmic Ca 2+ concentration.
    Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):57-71 PMID: 4144699
  9. A method for improving synchrony in the G2 phase of the cell cycle.
    J Cell Biol. 1971 Jun;49(3):942-5 PMID: 5092215
  10. Studies on cellular adhesion in tissue culture. XII. Some effects of cytochalasins and colchicine.
    Exp Cell Res. 1972 Sep;74(1):21-6 PMID: 4672474
  11. Cyclic AMP and calcium in relaxation in intestinal smooth muscle.
    Nat New Biol. 1972 Jul 26;238(82):119-20 PMID: 4339961
  12. Cytochalasin B II: selective inhibition of cytokinesis in Xenopus laevis eggs.
    Proc Soc Exp Biol Med. 1971 Apr;136(4):1158-62 PMID: 5102939
  13. Cytochalasin B. 3. Inhibition of human polymorphonuclear leukocyte phagocytosis.
    Proc Soc Exp Biol Med. 1971 May;137(1):161-4 PMID: 4932046
  14. Cytochalasin B: its morphological and electrophysiological actions on synthetic strands of cardiac muscle.
    Dev Biol. 1973 Apr;31(2):380-403 PMID: 4787205
  15. An analysis of salivary gland morphogenesis: role of cytoplasmic microfilaments and microtubules.
    Dev Biol. 1972 Jan;27(1):38-54 PMID: 5009494
  16. Cytochalasin B: lack of effect on mucopolysaccharide synthesis and selective alterations in precursor uptake.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2865-9 PMID: 4263507
  17. Effects of cytochalasins on mammalian cells.
    Nature. 1967 Jan 21;213(5073):261-4 PMID: 6067685
  18. Cytochalasin B: inhibition of glucose and glucosamine transport.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1430-4 PMID: 4338593
  19. Specialization of cell surfaces in contact-inhibited human glia-like cells in vitro.
    Exp Cell Res. 1971 Aug;67(2):407-15 PMID: 4328994
  20. Inhibition of the transport of several hexoses in mammalian cells by cytochalasin B.
    J Biol Chem. 1972 Jun 25;247(12):4102-5 PMID: 4338238
  21. Ultrastructure and function of growth cones and axons of cultured nerve cells.
    J Cell Biol. 1971 Jun;49(3):614-35 PMID: 4326456
  22. Growth control and cyclic alterations of cyclic AMP in the cell cycle.
    Nat New Biol. 1972 Oct 11;239(93):161-3 PMID: 4349672
  23. Changes in surface morphology of Chinese hamster ovary cells during the cell cycle.
    J Cell Biol. 1973 Jun;57(3):815-36 PMID: 4735453
  24. Ultrastructure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and "postfixation" in uranyl acetate.
    J Cell Biol. 1968 Sep;38(3):615-27 PMID: 4874495
  25. Adenosine triphosphate and motility of living systems.
    Harvey Lect. 1953-1954;49:37-56 PMID: 13232542
  26. Effects of cytochalasin B on polymorphonuclear leucocyte locomotion, phagocytosis and glycolysis.
    Exp Cell Res. 1972 Aug;73(2):383-93 PMID: 5054338
  27. Cytochalasin effects on BALB-3T3 fibroblasts: dose-dependent, reversible alteration of motility and cytoplasmic cleavage.
    Exp Cell Res. 1971 Sep;68(1):226-8 PMID: 5165445
  28. Scanning electron microscopy of cells in culture.
    Exp Cell Res. 1972;71(2):313-24 PMID: 4339895
  29. Cytochalasin B inhibits thyroid secretion.
    Biochem Biophys Res Commun. 1971 Jul 16;44(2):422-5 PMID: 4334139
  30. The effects of cytochalasin B on the microfilaments of baby hamster kidney (BHK-21) cells.
    J Cell Biol. 1972 Feb;52(2):246-54 PMID: 5061947
  31. Microfilaments in cellular and developmental processes.
    Science. 1971 Jan 15;171(3967):135-43 PMID: 5538822
  32. Neutrophilic leukocytes in immunologic reactions in vitro: effect of cytochalasin B.
    J Immunol. 1973 Jan;110(1):294-6 PMID: 4685406
  33. Cytochalasin B and release of growth hormone.
    Nat New Biol. 1971 Sep 15;234(50):215-6 PMID: 5288809
  34. Cytochalasin A and B. Inhibition of sugar uptake in cultured cells.
    J Biol Chem. 1972 May 10;247(9):2964-6 PMID: 4337109
  35. Junctions between cancer cells in culture: ultrastructure and permeability.
    Science. 1971 Nov 12;174(4010):717-9 PMID: 4330805
  36. The effects of cytochalasin D on differentiating muscle in culture.
    Tissue Cell. 1973;5(1):1-22 PMID: 4693988
  37. Cytochalasin B, its interaction with actin and actomyosin from muscle (cell movement-microfilaments-rabbit striated muscle).
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):442-6 PMID: 4258316
  38. [Adenosinetriphosphate as the energy substance for cell movement].
    Biochim Biophys Acta. 1954 Jun;14(2):182-94 PMID: 13172235
  39. Cyclic AMP levels in synchronized mammalian cells.
    Exp Cell Res. 1972 Nov;75(1):293-6 PMID: 4344159
  40. Cytochalasin B and the adenosine triphosphate content of treated fibroblasts.
    J Cell Biol. 1972 Oct;55(1):242-4 PMID: 4653417
  41. Effects of cytochalasin B on mammalian melanocytes and keratinocytes.
    J Invest Dermatol. 1972 Aug;59(2):163-9 PMID: 5050347
  42. Cytochalasin B: does it affect actin-like filaments?
    Science. 1972 Feb 18;175(4023):774-6 PMID: 4550685
  43. Membrane dynamics in the action of dibutyryl adenosine 3':5'-cyclic monophosphate and testosterone on mammalian cells.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1943-7 PMID: 4340166
  44. A unifying hypothesis of cell adhesion.
    Nature. 1966 Oct 22;212(5060):362-5 PMID: 4226267
  45. Enhancement of immunologically induced granule exocytosis from neutrophils by cytochalasin B.
    J Immunol. 1973 Jan;110(1):290-3 PMID: 4685405
  46. Role of contractile microfilaments in macrophage movement and endocytosis.
    Nat New Biol. 1971 Aug 4;232(31):153-5 PMID: 4937093
  47. Cytoplasmic filaments and morphogenesis: effects of cytochalasin B on contractile epidermal cells.
    Z Zellforsch Mikrosk Anat. 1972;132(2):167-92 PMID: 5083375
  48. Surface morphology of trypsinized human cells in vitro.
    Exp Cell Res. 1971 Jun;66(2):353-61 PMID: 4933742
  49. Intra- and extracellular control of epithelial morphogenesis.
    Symp Soc Dev Biol. 1970;29:195-249 PMID: 4125734
  50. Cytochalasin B: effects upon microfilaments involved in morphogenesis of estrogen-induced glands of oviduct.
    Proc Natl Acad Sci U S A. 1970 Jul;66(3):904-8 PMID: 5269252
  51. Cytochalasin blocks sympathetic ganglionic transmission: a presynaptic effect antagonized by pyruvate.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1730-3 PMID: 4352652
  52. Microfilaments and cell locomotion.
    J Cell Biol. 1971 Jun;49(3):595-613 PMID: 4933470
  53. Electronmicroscope investigations of the underside of cells in culture.
    Exp Cell Res. 1973 Mar 30;78(1):1-14 PMID: 4570581
  54. Cytochalasin B: inhibition of D-2-deoxyglucose transport into leukocytes and fibroblasts.
    Science. 1972 Jun 30;176(4042):1432-4 PMID: 5033651
  55. Cytochalasin D enhances infectivity of poliovirus.
    Proc Soc Exp Biol Med. 1973 Oct 1;144(1):60-4 PMID: 4358893
  56. STUDIES ON CELLULAR ADHESION IN TISSUE-CULTURE. 8. SOME EFFECTS OF ANTISERA ON CELL DETACHMENT.
    Exp Cell Res. 1965 Mar;37:540-51 PMID: 14315079
  57. Effects of cytochalasin B on muscle cells in tissue culture.
    Nat New Biol. 1971 Jan 27;229(4):121-3 PMID: 5285534
  58. Cytochalasin B. VI. Competitive inhibition of nucleoside transport by cultured Novikoff rat hepatoma cells.
    J Cell Biol. 1972 Oct;55(1):179-85 PMID: 4347250
  59. Cytochalasin B: effects on cell morphology, cell adhesion, and mucopolysaccharide synthesis (cultured cells-contractile microfilaments-glycoproteins-embryonic cells-sorting-out).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):253-7 PMID: 4257818
  60. Enucleation of mammalian cells by cytochalasin B. I. Characterization of anucleate cells.
    Exp Cell Res. 1972 Aug;73(2):273-86 PMID: 4626574
  61. Cytochalasin B: effect on lysosomal enzyme release from human leukocytes.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):844-8 PMID: 4351807
  62. Sub-lethal autolysis. Modification of cell periphery by lysosomal enzymes.
    Exp Cell Res. 1971 Jul;67(1):11-6 PMID: 4936354
  63. Binding of cytochalasin D to platelet and muscle myosin.
    J Biol Chem. 1973 Nov 25;248(22):7754-61 PMID: 4270769
  64. The inhibition of phagocytosis and facilitation of exocytosis in rabbit polymorphonuclear leukocytes by cytochalasin B.
    Lab Invest. 1973 Jan;28(1):16-22 PMID: 4569931
  65. Cytochalasin-B: time-lapse cinematographic studies on its effects on cytokinesis.
    J Cell Biol. 1972 Sep;54(3):657-64 PMID: 5044763
  66. The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella.
    Exp Cell Res. 1971 Aug;67(2):359-67 PMID: 5097522
  67. THE MECHANISM OF ADHESION OF CELLS TO GLASS. A STUDY BY INTERFERENCE REFLECTION MICROSCOPY.
    J Cell Biol. 1964 Feb;20:199-215 PMID: 14126869
  68. Cytochalasin B reversibly inhibits phagocytosis: functional, metabolic, and ultrastructural effects in human blood leukocytes and rabbit alveolar macrophages.
    Yale J Biol Med. 1971 Dec;44(3):286-300 PMID: 5132788
  69. Isolation and characterization of myosin from cloned mouse fibroblasts.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3693-7 PMID: 4264772
  70. Cytochalasin B: effect on hormone-mediated responses in cultured cells.
    J Cell Biol. 1973 Mar;56(3):857-61 PMID: 4405789
  71. Cytochalasin B. Dissociation of pinocytosis and phagocytosis by peritoneal macrophages.
    Exp Eye Res. 1973 Apr;79(1):73-8 PMID: 4736236
  72. Cytochalasin B. I. Effect on cytokinesis of Novikoff hepatoma cells.
    Proc Soc Exp Biol Med. 1971 Apr;136(4):1256-60 PMID: 4324358
  73. Electron microscopic identification of actin associated with isolated amoeba plasma membranes.
    J Biol Chem. 1973 Jan 25;248(2):448-50 PMID: 4567783
  74. Do morphogenetic tissue rearrangements require active cell movements? The reversible inhibition of cell sorting and tissue spreading by cytochalasin B.
    J Cell Biol. 1972 Dec;55(3):606-15 PMID: 4676369
  75. Stages of spreading of human diploid cells on glass surfaces.
    Exp Cell Res. 1971 Oct;68(2):372-80 PMID: 5124475
  76. Endocytosis in Chang liver cells. Quantitation by sucrose- 3 H uptake and inhibition by cytochalasin B.
    J Cell Biol. 1971 Sep;50(3):804-17 PMID: 4329157
  77. Haptotaxis and the mechanism of cell motility.
    Nature. 1967 Jan 21;213(5073):256-60 PMID: 6030602
  78. Inhibition of cell aggregation by antibodies directed against actomyosin.
    Nature. 1970 Apr 18;226(5242):261-2 PMID: 5461809
  79. Cell contact phenomena.
    In Vitro. 1970;5:48-78 PMID: 4941107
  80. Cytochalasin B-sensitive 2-deoxy-D-glucose transport in adipose cell ghosts.
    J Biol Chem. 1973 May 25;248(10):3636-41 PMID: 4735712
  81. The contractile ring. I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B.
    Z Zellforsch Mikrosk Anat. 1970;109(4):431-49 PMID: 5498229
  82. Contractile proteins of cultured cells. I. The isolation and characterization of an actin-like protein from cultured chick embryo fibroblasts.
    J Biol Chem. 1972 Jul 25;247(14):4503-9 PMID: 4261397
  83. [Microcinematographic and electron microscopic observations on cell surfaces and cell contacts in the human carcinoma cell culture line HEp 2].
    Virchows Arch B Cell Pathol. 1971;8(2):143-61 PMID: 4996729
  84. Microfilaments and cytoplasmic streaming: inhibition of streaming with cytochalasin.
    J Cell Sci. 1973 Jan;12(1):327-43 PMID: 4734741
  85. Failure of cytochalasin or colchicine to inhibit secretion of immunoglobulins.
    Nat New Biol. 1972 Feb 16;235(59):220-2 PMID: 4501537
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.61.2.481
Published
1974-05-00
Pages
481-500
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109298
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.61.2.481 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2