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PMID: 4126769 Published · ppublish English Journal Article

Homogenous rabbit 7S anti-IgG with antibody specificity for peptidoglycan.

The Journal of experimental medicine ·Vol. 138 ·No. 5 ·1973-11-01 ·Pages 1184-93

Bokisch VA, Chiao JW, Bernstein D, Krause RM

Abstract

The relationship between 7S anti-IgG and antibodies to streptococcal cell wall peptidoglycan was examined for four streptococcal Group C antisera. Homogeneous 7S anti-IgG components in these sera were isolated by means of an IgG immunoadsorbent column. For two of the four antisera, the anti-peptidoglycan activity of the 7S anti-IgG had specificity for the pentapeptide, L-Ala-D-Glu-gamma-L-Lys-D-Ala-D-Ala, the antigenic determinant of peptidoglycan, as well as for the Fc of IgG. Detailed studies on the 7S anti-IgG from one of the antisera revealed that the pentapeptide inhibited the coprecipitation reaction of 7S anti-IgG R3387 with antigen-antibody complexes and the precipitin reaction between 7S anti-IgG R3387 and its anti-idiotype serum.

MeSH Terms
Animals Antibodies, Anti-Idiotypic/analysis Antibody Specificity Antigen-Antibody Complex Binding Sites, Antibody Cell Wall/immunology Epitopes Immunoglobulin Fc Fragments Immunoglobulin G Peptides/pharmacology Peptidoglycan Precipitin Tests Rabbits/immunology Streptococcus/immunology
Chemicals
Antibodies, Anti-Idiotypic Antigen-Antibody Complex Epitopes Immunoglobulin Fc Fragments Immunoglobulin G Peptides Peptidoglycan
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bokisch V A
Chiao J W
Bernstein D
Krause R M
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18 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
Published
1973-11-01
Pages
1184-93
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139434
Subset
IM
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