Polycistronic effects of catabolite repression on the lac operon.

Journal of bacteriology ·Vol. 112 ·No. 3 ·1972-12-00 ·Pages 1184-92

Silverstone AE, Magasanik B

Abstract

The catabolite repression caused by glucose and glucose-6-phosphate has been studied for both beta-galactosidase and thiogalactoside transacetylase, the products of the operator proximal and distal cistrons of the lac operon, respectively. We find that both cistrons are affected coordinately by this form of repression. We also find that a single alteration at the lac promoter region is sufficient to abolish sensitivity to repression of both cistrons. From this, we conclude that there is only one target site for catabolite repression in the lac operon.

MeSH Terms

Acetyltransferases/biosynthesis Chloramphenicol/pharmacology Chromosome Mapping Chromosomes, Bacterial Coliphages Dactinomycin/pharmacology Edetic Acid Enzyme Induction/drug effects Enzyme Repression Escherichia coli/enzymology,metabolism Galactose Galactosidases/biosynthesis Glucose/pharmacology Glucosephosphates/pharmacology Glycosides Lactose/metabolism Operon Protein Biosynthesis RNA, Messenger/biosynthesis Rifampin/pharmacology Transcription, Genetic Transduction, Genetic Tryptophan/biosynthesis

Chemicals

Glucosephosphates Glycosides RNA, Messenger Dactinomycin Chloramphenicol Tryptophan Edetic Acid Acetyltransferases Galactosidases Glucose Lactose Rifampin Galactose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Silverstone A E

Magasanik B

References (25)

25 references, click to expand

Molecular basis of transient repression of beta-galactosidase in Escherichia coli.
J Bacteriol. 1969 Feb;97(2):550-6 PMID: 4886283
Catabolite sensitive site of the lac operon.
Nature. 1969 Mar 15;221(5185):1012-4 PMID: 4886742
THE RELATION OF CATABOLITE REPRESSION TO THE INDUCTION SYSTEM FOR BETA-GALACTOSIDASE IN ESCHERICHIA COLI.
J Mol Biol. 1964 Mar;8:417-26 PMID: 14168695
Operon coordination in different bacterial hosts.
J Bacteriol. 1970 Aug;103(2):318-22 PMID: 4914562
Catabolite repression in Escherichia coli. A study of two hypotheses.
Biochem J. 1968 Nov;110(1):135-42 PMID: 4881142
Mechanism of beta-galactosidase induction in Escherichia coli.
J Mol Biol. 1967 Aug 14;27(3):475-94 PMID: 4860580
The step sensitive to catabolite repression and its reversal by 3'-5' cyclic AMP during induced synthesis of beta-galactosidase in E. coli.
Biochem Biophys Res Commun. 1969 Jul 7;36(1):84-92 PMID: 4307746
Interaction of prophages at the att80 site with the chromosome of Escherichia coli.
J Mol Biol. 1966 Jan;15(1):243-55 PMID: 5330218
Fusions of the lac and trp Regions of the Escherichia coli Chromosome.
J Bacteriol. 1970 Dec;104(3):1273-9 PMID: 16559103
Carabolite repression of the lac operon. Repression of translation.
Biochem J. 1969 Jun;113(2):423-8 PMID: 4897202
Catabolite repression gene of Escherichia coli.
J Bacteriol. 1969 Nov;100(2):809-16 PMID: 4901362
The gradient of polarity of z gene nonsense mutations in trp-lac fusion strains of Escherichia coli.
J Mol Biol. 1971 Jan 14;55(1):119-22 PMID: 4926973
The effect of catabolite repression and of cyclic 3',5' adenosine monophosphate on the translation of the lactose messenger RNA in Escherichia coli.
Biochem Biophys Res Commun. 1970 Mar 27;38(6):1023-32 PMID: 4314388
Catabolite repression of the lac operon. The contribution f trascriptional repression.
Biochem J. 1969 Sep;114(2):307-11 PMID: 4897462
Catabolite-insensitive revertants of lac promoter mutants.
Proc Natl Acad Sci U S A. 1970 Jul;66(3):773-9 PMID: 4913210
The non-linear relationship between the enzyme activity and structural protein concentration of thiogalactoside transacetylase of E. coli.
Biochem Biophys Res Commun. 1969 Feb 21;34(4):522-7 PMID: 4887461
Nonsense mutants and polarity in the lac operon of Escherichia coli.
J Mol Biol. 1965 Nov;14(1):290-6 PMID: 5327654
A mechanism for repressor action.
J Mol Biol. 1969 Jul 14;43(1):201-13 PMID: 4897791
Sequential transcription and translation in the lactose operon of Escherichia coli.
Biochim Biophys Acta. 1967 Mar 29;138(1):107-23 PMID: 4860427
THE ROLES OF INDUCER AND CATABOLITE REPRESSOR IN THE SYNTHESIS OF BETA-GALACTOSIDASE BY ESCHERICHIA COLI.
J Mol Biol. 1964 Jan;8:105-27 PMID: 14149954
Transposition of the lac region of Escherichia coli. II. On the role of thiogalactoside transacetylase in lactose metabolism.
J Mol Biol. 1966 Aug;19(2):576-9 PMID: 5338859
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
THE ORDER OF INDUCTION AND DEINDUCTION OF THE ENZYMES OF THE LACTOSE OPERON IN E. COLI.
Proc Natl Acad Sci U S A. 1965 Apr;53:797-802 PMID: 14324536
Synthesis, utilization and degradation of lactose operon mRNA in Escherichia coli.
J Mol Biol. 1967 Mar 14;24(2):247-59 PMID: 4961803
Genetic evidence that the operator locus is distinct from the the z gene in the lac operon of Escherichia coli.
J Mol Biol. 1969 Jul 14;43(1):215-8 PMID: 4897792

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.112.3.1184-1192.1972

Published

1972-12-00

Pages

1184-92

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251547

Subset

External Links

PubMed source DOI: 10.1128/jb.112.3.1184-1192.1972 PMC Full Text View journal details

Analysis Services

Polycistronic effects of catabolite repression on the lac operon.

Contact

WeChat Official Account