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PMID: 4065096 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The structure of the human c-fes/fps proto-oncogene.

The EMBO journal ·Vol. 4 ·No. 11 ·1985-11-00 ·Pages 2897-903

Roebroek AJ, Schalken JA, Verbeek JS, Van den Ouweland AM, Onnekink C, Bloemers HP, Van de Ven WJ

Abstract

We have determined the complete nucleotide sequence of a human DNA fragment of approximately 13 kbp, which was shown by Southern blot analysis to contain the entire v-fes/fps cellular homolog. The v-fes/fps homologous sequences were dispersed over 11 kbp in 18 interspersed segments which were flanked by splice junctions. Fusion of these segments created a DNA fragment in which coding regions similar to those observed in the viral oncogenes v-fes of the Gardner-Arnstein (GA) and Snyder-Theilen (ST) strains of feline sarcoma virus and v-fps found in Fujinami sarcoma virus could be identified. A potential initiation site in the first exon was found. About 200 nucleotides downstream of a translational stop codon in the v-fes/fps homologous region, a poly(A) addition signal was identified. The deduced amino acid sequence has a molecular weight of 93 390 dalton resembling NCP92, the recently described human c-fes/fps product. The topography of human c-fes/fps appeared to resemble that of chicken c-fps.

MeSH Terms
Amino Acid Sequence Animals Base Composition Base Sequence Chickens Cosmids Humans Nucleic Acid Hybridization Proto-Oncogene Mas Proto-Oncogenes Sequence Homology, Nucleic Acid
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Roebroek A J
Schalken J A
Verbeek J S
Van den Ouweland A M
Onnekink C
Bloemers H P
Van de Ven W J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-11-00
Pages
2897-903
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554595
Subset
IM
Databases
GENBANK
X06292
External Links
PubMed source PMC Full Text View journal details
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