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PMID: 4015627 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Induction of metallothionein mRNA in rat liver and kidney after copper chloride injection.

The Biochemical journal ·Vol. 228 ·No. 2 ·1985-06-01 ·Pages 425-32

Wake SA, Mercer JF

Abstract

The kinetics of the increase of metallothionein mRNA in rat liver and kidney after CuCl2 injection was determined by cell-free translation and dot-blot hybridization of total RNA isolated at various times after the injection. Both assay procedures gave essentially the same result: a 16-fold increase in hepatic metallothionein mRNA was observed 7h after CuCl2 injection, with a decline to basal values by 15 h. The response in the kidney was less dramatic, with a 6-fold increase in metallothionein mRNA 5 h after injection, and basal values were attained by 12h. The rise in Cu2+ concentration in both organs was closely correlated with the increase in metallothionein mRNA; hepatic Cu2+ was increased 5.9-fold by 5h after injection and renal Cu2+ was increased 4.3-fold 5h after injection. The Zn2+ concentration in the liver had not risen significantly within 5h of Cu2+ injection. Renal Zn2+ concentrations did not alter appreciably in the Cu2+-treated animals. These results support the conclusion that Cu2+ is acting as a primary inducer of metallothionein mRNA in the rat.

MeSH Terms
Adrenalectomy Animals Copper/pharmacology Kidney/drug effects,metabolism Liver/drug effects,metabolism Male Metallothionein/genetics,metabolism Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/biosynthesis,genetics Rats Rats, Inbred Strains Sulfates/pharmacology Zinc/pharmacology Zinc Sulfate
Chemicals
RNA, Messenger Sulfates Zinc Sulfate Copper Metallothionein cuprous chloride Zinc
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wake S A
Mercer J F
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22 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2280425
Published
1985-06-01
Pages
425-32
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1145000
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2280425 PMC Full Text View journal details
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