Nalidixic acid causes a transient G1 arrest in the yeast Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 176 ·No. 1 ·1979-10-02 ·Pages 37-9

Singer RA, Johnston GC

Abstract

The addition of nalidixic acid to growing cells of the yeast Saccharomyces cerevisiae resulted in a transient depression in the rate of ribosomal precursor RNA production and a transient arrest of cells in G1. Protein synthesis rates were less affected. Lower concentrations of nalidixic acid also affected RNA synthesis and progression through G1 but had no effect on protein synthesis rates. We suggest that nalidixic acid has a primary effect on RNA synthesis leading to a G1 arrest.

MeSH Terms

Cell Cycle/drug effects Nalidixic Acid/pharmacology Nucleic Acid Precursors/biosynthesis RNA, Fungal/biosynthesis RNA, Ribosomal/biosynthesis Saccharomyces cerevisiae/drug effects

Chemicals

Nucleic Acid Precursors RNA, Fungal RNA, Ribosomal Nalidixic Acid

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Singer R A

Johnston G C

References (10)

10 references, click to expand

Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase.
Nature. 1978 Oct 5;275(5679):420-3 PMID: 211433
Growth and cell division during nitrogen starvation of the yeast Saccharomyces cerevisiae.
J Bacteriol. 1977 Nov;132(2):723-30 PMID: 334751
Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis.
J Mol Biol. 1974 Apr 15;84(3):445-61 PMID: 4618856
Effect of putative deoxyribonucleic acid inhibitors on macromolecular synthesis in Saccharomyces cerevisiae.
J Bacteriol. 1970 Jun;102(3):636-41 PMID: 4246878
Saccharomyces cerevisiae cell cycle.
Bacteriol Rev. 1974 Jun;38(2):164-98 PMID: 4599449
Studies on the action of nalidixic acid in the yeast Saccharomyces cerevisiae.
Antimicrob Agents Chemother. 1973 May;3(5):562-7 PMID: 4597731
RNA synthesis and control of cell division in the yeast S. cerevisiae.
Cell. 1978 Aug;14(4):951-8 PMID: 99247
Inhibition by nalidixic acid of nucleic acid and protein synthesis in Saccharomyces cerevisiae [proceedings].
Biochem Soc Trans. 1977;5(5):1503-5 PMID: 336436
An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate.
J Chromatogr. 1969 Mar 11;40(1):103-9 PMID: 5777609
Methionine analogs and cell division regulation in the yeast Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6083-7 PMID: 366609

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00334293

Published

1979-10-02

Pages

37-9

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00334293

Analysis Services

Nalidixic acid causes a transient G1 arrest in the yeast Saccharomyces cerevisiae.

Contact

WeChat Official Account