Abstract
Extracellular production of glucoamylase from the yeast Saccharomyces diastaticus carrying an STA1 gene (a structural gene for the enzyme) is repressed by the mating-type constitution a/alpha. We obtained mutants that were relieved from the mating-type control of the STA1 gene expression. Tetrad analysis showed that the mutation was closely linked to MATa. The mutation was complemented by a MATa2 gene; hence, it was designated mata2. Northern blot analysis revealed that the MATa2-mediated repression occurs at the posttranscriptional level.
MeSH Terms
Enzyme Repression
Gene Expression Regulation
Genes
Genes, Fungal
Genes, Mating Type, Fungal
Genes, Regulator
Genetic Complementation Test
Genetic Linkage
Glucan 1,4-alpha-Glucosidase/biosynthesis,genetics
Glucosidases/genetics
Mutation
Saccharomyces cerevisiae/enzymology,genetics,physiology
Transcription, Genetic
Chemicals
Glucosidases
Glucan 1,4-alpha-Glucosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yamashita I
Takano Y
Fukui S
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