Home LiteratureArticle Details
PMID: 3903748 Published · ppublish English Journal Article

Synthesis and secretion of human epidermal growth factor by Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 21 ·1985-11-00 ·Pages 7212-6

Oka T, Sakamoto S, Miyoshi K, Fuwa T, Yoda K, Yamasaki M, Tamura G, Miyake T

Abstract

A synthetic gene for human epidermal growth factor (hEGF) was joined to a sequence encoding the signal peptide of Escherichia coli alkaline phosphatase. This hybrid gene was placed under the control of the alkaline phosphatase gene (phoA) promoter in a recombinant plasmid, which was used to transfect E. coli. The hybrid protein that was expressed in host cells under conditions of phosphate limitation was processed accurately during the secretion process, and mature hEGF was recovered in the periplasmic fraction. On the other hand, no EGF was detected in the periplasmic space when the synthetic hEGF gene was not accompanied by the phoA signal sequence.

MeSH Terms
Alkaline Phosphatase/genetics Amino Acid Sequence Bacterial Proteins/genetics Epidermal Growth Factor/biosynthesis,genetics,metabolism Escherichia coli/genetics,metabolism Gene Expression Regulation Genes, Synthetic Humans Promoter Regions, Genetic Protein Processing, Post-Translational Protein Sorting Signals/genetics Recombinant Proteins/genetics,metabolism
Chemicals
Bacterial Proteins Protein Sorting Signals Recombinant Proteins Epidermal Growth Factor Alkaline Phosphatase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Oka T
Sakamoto S
Miyoshi K
Fuwa T
Yoda K
Yamasaki M
Tamura G
Miyake T
References (30)
30 references, click to expand
  1. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts.
    J Biol Chem. 1965 Sep;240(9):3685-92 PMID: 4284300
  2. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.
    Biochim Biophys Acta. 1960 Mar 11;38:470-83 PMID: 13826559
  3. Effect of urogastrone in the Zollinger-Ellison syndrome.
    Lancet. 1975 Sep 6;2(7932):424-7 PMID: 51236
  4. Isolation and structure of urogastrone and its relationship to epidermal growth factor.
    Nature. 1975 Sep 25;257(5524):325-7 PMID: 1161035
  5. Effect of urogastrone on gastric secretion and plasma gastrin levels in normal subjects.
    Gut. 1975 Nov;16(11):887-93 PMID: 1104412
  6. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
    J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
  7. Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1440-4 PMID: 323853
  8. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.
    Science. 1977 Dec 9;198(4321):1056-63 PMID: 412251
  9. Epidermal growth factor stimulates phosphorylation in membrane preparations in vitro.
    Nature. 1978 Nov 23;276(5686):409-10 PMID: 309559
  10. The assembly of proteins into biological membranes: The membrane trigger hypothesis.
    Annu Rev Biochem. 1979;48:23-45 PMID: 224802
  11. Trans-membrane translocation of proteins. The direct transfer model.
    Eur J Biochem. 1979 Jun;97(1):175-81 PMID: 477664
  12. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  13. Bacteria mature preproinsulin to proinsulin.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3988-92 PMID: 7001451
  14. Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.
    Nucleic Acids Res. 1980 Nov 25;8(22):5473-89 PMID: 7465418
  15. Protein localization in E. coli: is there a common step in the secretion of periplasmic and outer-membrane proteins?
    Cell. 1981 Jun;24(3):707-17 PMID: 6788377
  16. An 'internal' signal sequence directs secretion and processing or proinsulin in bacteria.
    Nature. 1981 Nov 12;294(5837):176-8 PMID: 7029306
  17. The nucleotide sequence of the promoter and the amino-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli.
    Nucleic Acids Res. 1981 Nov 11;9(21):5671-8 PMID: 6273802
  18. Proteases in Escherichia coli.
    Methods Enzymol. 1981;80 Pt C:680-702 PMID: 7043205
  19. Cellular location affects protein stability in Escherichia coli.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1830-3 PMID: 7043465
  20. A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5200-4 PMID: 6291030
  21. Secretion of human interferons by yeast.
    Science. 1983 Feb 11;219(4585):620-5 PMID: 6186023
  22. Expression of chemically synthesized alpha-neo-endorphin gene fused to E. coli alkaline phosphatase.
    Nucleic Acids Res. 1983 Mar 11;11(5):1283-94 PMID: 6298740
  23. Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursor.
    Nature. 1983 Jun 23-29;303(5919):722-5 PMID: 6304537
  24. The role of the beta-lactamase signal sequence in the secretion of proteins by Escherichia coli.
    J Biol Chem. 1984 Feb 25;259(4):2149-54 PMID: 6365904
  25. Compilation of published signal sequences.
    Nucleic Acids Res. 1984 Jul 11;12(13):5145-64 PMID: 6379599
  26. Human transforming growth factor-alpha: precursor structure and expression in E. coli.
    Cell. 1984 Aug;38(1):287-97 PMID: 6088071
  27. Secretion cloning vectors in Escherichia coli.
    EMBO J. 1984 Oct;3(10):2437-42 PMID: 6094184
  28. Secretion of human interferon-alpha induced by using secretion vectors containing a promoter and signal sequence of alkaline phosphatase gene of Escherichia coli.
    J Biochem. 1985 May;97(5):1429-36 PMID: 3928609
  29. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli.
    Biochim Biophys Acta. 1960 Mar 11;38:460-9 PMID: 13838951
  30. The release of enzymes by osmotic shock from Escherichia coli in exponential phase.
    J Biol Chem. 1966 Jul 10;241(13):3055-62 PMID: 4287907
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.21.7212
Published
1985-11-00
Pages
7212-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390819
Subset
IM
Databases
GENBANK
M11936
External Links
PubMed source DOI: 10.1073/pnas.82.21.7212 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2