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PMID: 6291030 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 17 ·1982-09-00 ·Pages 5200-4

Boeke JD, Model P

Abstract

Gene III protein of bacteriophage f1 is inserted into the host cell membrane where it is assembled into phage particles. A truncated form of gene III protein, encoded by a recombinant plasmid and lacking the carboxyl terminus, does not remain in the membrane but instead appears to slip through it. Fusion of a hydrophobic "membrane anchor" from another membrane protein, the gene VIII protein, to the truncated gene III protein (by manipulation of the recombinant plasmid) restores membrane anchoring. A model for the relationship of gene III protein with the Escherichia coli membrane is discussed.

MeSH Terms

Cell Membrane/physiology Cloning, Molecular Coliphages/genetics DNA Restriction Enzymes Escherichia coli/genetics Genes, Viral Plasmids Viral Proteins/genetics

Chemicals

Viral Proteins DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Boeke J D

Model P

References (26)

26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.17.5200

Published

1982-09-00

Pages

5200-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC346863

Subset

External Links

PubMed source DOI: 10.1073/pnas.79.17.5200 PMC Full Text View journal details

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A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane.

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