Home LiteratureArticle Details
PMID: 3892481 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence that pheV, a gene for tRNAPhe of E. coli is transcribed from tandem promoters.

Nucleic acids research ·Vol. 13 ·No. 10 ·1985-05-24 ·Pages 3699-710

Caillet J, Plumbridge JA, Springer M

Abstract

A DNA fragment of 487 bp containing a gene for tRNAPhe has been sequenced. Although the tRNAPhe sequence is identical to that of pheU (which maps at 94.5 min) the surrounding sequences are quite different. This sequence is thus that of a second gene for tRNAPhe (which we shall call pheV). In vitro transcription experiments and S1 mapping in vivo show the existence of two promoters separated by about 60 nucleotides. The second transcript starts only 3 nucleotides 5' from the tRNAPhe structural sequence. A DNA sequence characteristic of a rho-independent terminator is located 30 nucleotides 3' of the end of the structural gene and is shown to function efficiently in vitro.

MeSH Terms
Base Sequence Escherichia coli/genetics Genes Genes, Bacterial In Vitro Techniques Operon Plasmids RNA, Transfer, Amino Acyl/genetics Transcription, Genetic
Chemicals
RNA, Transfer, Amino Acyl
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Caillet J
Plumbridge J A
Springer M
References (22)
22 references, click to expand
  1. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  2. Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331-5 PMID: 6158044
  3. Organization of transfer ribonucleic acid genes in the Escherichia coli chromosome.
    J Bacteriol. 1980 Dec;144(3):1083-93 PMID: 6160141
  4. Organization and structure of an E. coli tRNA operon containing seven tRNA genes.
    Cell. 1981 Jan;23(1):239-49 PMID: 6163550
  5. Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1421-5 PMID: 6165011
  6. Nucleotide sequence of an Escherichia coli tRNA (Leu 1) operon and identification of the transcription promoter signal.
    Nucleic Acids Res. 1981 May 11;9(9):2121-39 PMID: 6272226
  7. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  8. Identification of clones carrying an E. coli tRNAPhe gene by suppression of phenylalanyl-tRNA synthetase thermosensitive mutants.
    Nucleic Acids Res. 1983 Feb 11;11(3):727-36 PMID: 6300764
  9. Differential stringent control of the tandem E. coli ribosomal RNA promoters from the rrnA operon expressed in vivo in multicopy plasmids.
    Cell. 1983 Apr;32(4):1337-46 PMID: 6188537
  10. Regions of DNA involved in the stringent control of plasmid-encoded rRNA in vivo.
    Cell. 1983 Apr;32(4):1347-54 PMID: 6188538
  11. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  12. Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe.
    Nucleic Acids Res. 1983 Jul 11;11(13):4379-89 PMID: 6306588
  13. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  14. tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):409-13 PMID: 6364142
  15. Conserved features of coordinately regulated E. coli promoters.
    Nucleic Acids Res. 1984 Mar 26;12(6):2605-18 PMID: 6369249
  16. Sequence of a 1.26-kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon.
    EMBO J. 1982;1(3):311-5 PMID: 6325158
  17. Genetic mapping of pheU, an Escherichia coli gene for phenylalanine tRNA.
    J Bacteriol. 1984 May;158(2):762-3 PMID: 6373735
  18. Structure of an Escherichia coli tRNA operon containing linked genes for arginine, histidine, leucine, and proline tRNAs.
    J Bacteriol. 1984 Jun;158(3):934-42 PMID: 6327651
  19. Regulation of the synthesis of ribosomes and ribosomal components.
    Annu Rev Biochem. 1984;53:75-117 PMID: 6206783
  20. Identification of transfer RNA suppressors in Escherichia coli. III. Ochre suppressors of lysine tRNA.
    J Mol Biol. 1984 Aug 25;177(4):609-25 PMID: 6207301
  21. Escherichia coli tyrosine transfer ribonucleic acid genes. Nucleotide sequences of their promoters and of the regions adjoining C-C-A ends.
    J Biol Chem. 1976 Sep 10;251(17):5124-40 PMID: 821949
  22. Construction of plasmids carrying the cI gene of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174-8 PMID: 1069307
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.10.3699
Published
1985-05-24
Pages
3699-710
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341267
Subset
IM
Databases
GENBANK
X02480
External Links
PubMed source DOI: 10.1093/nar/13.10.3699 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2