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PMID: 6364142 Published · ppublish English Journal Article

tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 2 ·1984-01-00 ·Pages 409-13

Ishii S, Kuroki K, Imamoto F

Abstract

The promoter-proximal portion of the operon containing the Escherichia coli nusA gene has been cloned. Its nucleotide sequence shows that genes for tRNAMetf2 and a 15-kilodalton protein of unknown function precede the nusA protein gene. The sequence suggests that the three genes form a single transcription unit. Consistent with this hypothesis, purified RNA polymerase formed full-length transcripts on the cloned DNA in vitro, although transcription was frequently arrested at the intercistronic site(s) between the gene for tRNAMetf2 and the 15-kilodalton protein.

MeSH Terms
Bacterial Proteins/genetics Base Sequence DNA, Bacterial/genetics Escherichia coli/genetics Gene Expression Regulation Genes Molecular Weight Operon RNA, Transfer/genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial Transcription Factors RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ishii S
Kuroki K
Imamoto F
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.2.409
Published
1984-01-00
Pages
409-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344686
Subset
IM
Databases
GENBANK
K01175, X00513
External Links
PubMed source DOI: 10.1073/pnas.81.2.409 PMC Full Text View journal details
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