Interaction between RNA polymerase and a ribosomal RNA promoter of E. coli.

Nucleic acids research ·Vol. 7 ·No. 4 ·1979-10-25 ·Pages 1019-33

Hamming J, Gruber M, AB G

Abstract

The interaction between RNA polymerase and the E. coli ribosomal (r) RNA promoter(s) of the rrnE operon has been studied by the filter-binding method. The extent of complex formation between RNA polymerase and rrnE promoter(s) is salt-dependent; ppGpp specifically inhibits interaction of RNA polymerase with the rrnE promoter(s). A tentative model is proposed for the molecular events in the early steps of rRNA initiation: a transition of the primarily formed, labile RNA polymerase-rRNA promoter complex to a more stable form is the determining step. This step is salt-sensitive; ppGpp acts on this "isomerization".

MeSH Terms

DNA-Directed RNA Polymerases/metabolism Drug Stability Escherichia coli/metabolism Guanosine Tetraphosphate/pharmacology Kinetics Operon Protein Binding RNA, Ribosomal/metabolism Transcription, Genetic

Chemicals

RNA, Ribosomal Guanosine Tetraphosphate DNA-Directed RNA Polymerases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hamming J

Gruber M

AB G

References (23)

23 references, click to expand

Isolation of lambda transducing phages carrying rRNA genes at the metA-purD region of the Escherichia coli chromosome.
FEBS Lett. 1976 Dec 31;72(2):256-61 PMID: 16386035
A method for the recovery of DNA from agarose gels.
Nucleic Acids Res. 1978 Jul;5(7):2321-32 PMID: 673856
A physical map of the DNA regions flanking the rabbit beta-globin gene.
Cell. 1977 Oct;12(2):429-39 PMID: 912752
RNA polymerase interaction at the promoter--operator region of the tryptophan operon of Escherichia coli and Salmonella typhimurium.
J Mol Biol. 1978 May 15;121(2):153-77 PMID: 351193
Interaction of RNA polymerase with promoters from bacteriophage fd.
Eur J Biochem. 1977 Mar 15;74(1):107-13 PMID: 300680
Positive control of lac operon expression in vitro by guanosine 5'-diphosphate 3'-diphosphate.
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1726-30 PMID: 109832
Tandem promoters direct E. coli ribosomal RNA synthesis.
Cell. 1979 May;17(1):225-34 PMID: 110460
In vitro transcripts from the rrn B ribosomal RNA cistron originate from two tandem promoters.
Cell. 1979 Jan;16(1):111-21 PMID: 369703
Identification of initiation sites for the in vitro transcription of rRNA operons rrnE and rrnA in E. coli.
Cell. 1979 May;17(1):211-24 PMID: 378406
Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5 PMID: 368799
The sequence of Escherichia coli ribosomal 16 S RNA determined by new rapid gel methods.
FEBS Lett. 1978 Oct 1;94(1):152-6 PMID: 359355
Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli.
J Bacteriol. 1977 Dec;132(3):931-49 PMID: 336613
The number of rRNA genes in Escherichia coli.
FEBS Lett. 1977 Jul 1;79(1):77-9 PMID: 330226
In vitro transcription of three different ribosomal RNA cistrons of E. coli; heterogeneity of control regions.
Mol Gen Genet. 1977 Mar 28;152(1):1-6 PMID: 325380
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
Modulation of RNA polymerase specificity by ppGpp.
Mol Gen Genet. 1976 Aug 19;147(2):225-32 PMID: 787760
Construction of plasmids carrying the cI gene of bacteriophage lambda.
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174-8 PMID: 1069307
A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
Specific inhibition of ribosomal RNA synthesis in vitro by guanosine 3' diphosphate, 5' diphosphate.
Nature. 1975 Apr 10;254(5500):530-1 PMID: 1091863
The mechanism of action of ppGpp on rRNA synthesis in vitro.
Cell. 1976 May;8(1):123-8 PMID: 8211
Chromatography of rNA polymerase from escherichia coli on single stranded DNA-agarose columns.
Biochem Biophys Res Commun. 1972 Apr 14;47(1):282-9 PMID: 4554814
The control of ribonucleic acid synthesis in Escherichia coli. IV. Relevance of unusual phosphorylated compounds from amino acid-starved stringent strains.
J Biol Chem. 1969 Jun 25;244(12):3133-41 PMID: 4893338
In vitro synthesis of the E3 immunity protein directed by Col E3 plasmid deoxyribonucleic acid.
J Biol Chem. 1975 Feb 10;250(3):1123-31 PMID: 803499

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/7.4.1019

Published

1979-10-25

Pages

1019-33

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342279

Subset

External Links

PubMed source DOI: 10.1093/nar/7.4.1019 PMC Full Text View journal details

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Interaction between RNA polymerase and a ribosomal RNA promoter of E. coli.

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