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PMID: 3881768 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human major histocompatibility complex class I antigens: residues 61-83 of the HLA-B7 heavy chain specify an alloreactive site.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 2 ·1985-01-00 ·Pages 539-42

Walker LE, Ketler TA, Houghten RA, Schulz G, Chersi A, Reisfeld RA

Abstract

A chemically synthesized peptide (sequence in text) homologous to residues 61-83 of the HLA-B7 heavy chain, induced antibodies that specifically recognized the HLA heavy chain-beta 2-microglobulin complex and the free heavy chain of the HLA-B7 antigen. These antibodies specifically immunoprecipitated the HLA-B7 beta 2-microglobulin complex solubilized from human lymphoblastoid cells by nonionic detergents and reacted with free HLA-B7 heavy chains in blots on nitrocellulose. These observations suggest that the antigenic conformation of this region of the HLA-B7 molecule is independent of the presence of beta 2-microglobulin and that amino acid residues 61-83 mimic an alloreactive site expressed by the HLA-B7 antigen.

MeSH Terms

Amino Acid Sequence Antibody-Dependent Cell Cytotoxicity Cell Line HLA Antigens/analysis,immunology HLA-B Antigens Humans Isoantibodies/immunology Phenotype Structure-Activity Relationship beta 2-Microglobulin/immunology

Chemicals

HLA Antigens HLA-B Antigens HLA-B17 antigen Isoantibodies beta 2-Microglobulin

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Walker L E

Ketler T A

Houghten R A

Schulz G

Chersi A

Reisfeld R A

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.2.539

Published

1985-01-00

Pages

539-42

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC397075

Subset

Grants

NIAID NIH HHS · AI 10180 · United States

External Links

PubMed source DOI: 10.1073/pnas.82.2.539 PMC Full Text View journal details

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Human major histocompatibility complex class I antigens: residues 61-83 of the HLA-B7 heavy chain specify an alloreactive site.

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