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PMID: 3881766 Published · ppublish English Journal Article

The Ly-15 alloantigenic system: a genetically determined polymorphism of the murine lymphocyte function-associated antigen-1 molecule.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 2 ·1985-01-00 ·Pages 526-30

Hogarth PM, Walker ID, McKenzie IF, Springer TA

Abstract

Serological and biochemical studies using monoclonal antibodies have demonstrated that the Ly-15 cell membrane alloantigens are polymorphic sites on the lymphocyte function-associated antigen-1 (LFA-1) molecule. Ly-15.2 and LFA-1 show identical tissue distributions, being present on all thymocytes, lymphocytes, and neutrophils, and flow cytofluorometric analysis indicated identical cell surface expression of these molecules. Identity of Ly-15.2 and LFA-1 was confirmed by immunochemical analysis. The Ly-15.2 and LFA-1 molecules have an identical heterodimeric structure of Mr 180,000 alpha chain and Mr 94,000 beta chain, which coelectrophorese on two-dimensional NaDodSO4/PAGE. Furthermore, anti-Ly-15.2 and anti-LFA-1 antibodies coprecipitate the same molecule from thymocyte lysates, and peptide mapping studies show that the Ly-15.2 and LFA-1 alpha chains are identical, as are the beta chains.

MeSH Terms
Animals Antigens, Surface/genetics Electrophoresis, Polyacrylamide Gel Flow Cytometry Immunosorbent Techniques Isoantigens/genetics Lymphocyte Function-Associated Antigen-1 Mice Mice, Inbred Strains Molecular Weight Polymorphism, Genetic Species Specificity Tissue Distribution
Chemicals
Antigens, Surface Isoantigens Lymphocyte Function-Associated Antigen-1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hogarth P M
Walker I D
McKenzie I F
Springer T A
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.2.526
Published
1985-01-00
Pages
526-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397072
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.2.526 PMC Full Text View journal details
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