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PMID: 3881401 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Repressor for the sn-glycerol-3-phosphate regulon of Escherichia coli K-12: cloning of the glpR gene and identification of its product.

Journal of bacteriology ·Vol. 161 ·No. 2 ·1985-02-00 ·Pages 563-6

Schweizer H, Boos W, Larson TJ

Abstract

The glpR gene encoding the repressor for the glp regulon of Escherichia coli was cloned from a library of HindIII DNA fragments established in bacteriophage lambda. Phages harboring glpR were isolated by selection for sn-glycerol-3-phosphate dehydrogenase function encoded by glpD, which is adjacent to glpR on the E. coli linkage map. Restriction endonuclease analysis and recloning of DNA fragments localized glpR to a 3-kilobase-pair EcoRI-SalI segment of DNA. Strains exhibiting constitutive expression of the glp operons were strongly repressed after introduction of multicopy plasmids containing the glpR gene. Analysis of proteins labeled in minicells harboring either glpR+ recombinant plasmids or a glpR::Tn5 derivative showed that the glpR gene product is a protein with an apparent molecular weight of 33,000.

MeSH Terms
Cloning, Molecular Coliphages/genetics Escherichia coli/genetics,metabolism Genes, Regulator Glycerophosphates/genetics Transduction, Genetic
Chemicals
Glycerophosphates alpha-glycerophosphoric acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schweizer H
Boos W
Larson T J
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19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.2.563-566.1985
Published
1985-02-00
Pages
563-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214919
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.161.2.563-566.1985 PMC Full Text View journal details
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