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PMID: 6392822 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the ugp region containing the genes for the phoB dependent sn-glycerol-3-phosphate transport system of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 197 ·No. 1 ·1984-00-00 ·Pages 161-8

Schweizer H, Boos W

Abstract

The ugp structural genes, coding for the pho regulon dependent sn-glycerol-3-phosphate transport system, were cloned in pBR322 and characterized. The expression of the cloned ugp system was phoB dependent. Cells containing the ugp plasmid overproduced the G3P binding protein upon phosphate starvation. Tn5 mutagenesis of the cloned DNA revealed that the ugp genes are organized in two separate operons which comprise at least four genes: ugpB and ugpD constitute one operon, ugpA and ugpC constitute the other. The structural gene for the G3P binding protein (G3PBP) is ugpB. The ugpC gene product was also synthesized in minicells as a polypeptide, with an apparent molecular weight of 40,000. No gene products could be assigned to the ugpA and ugpD genes. Hybridization experiments allowed the physical characterization of 20 kb of DNA adjacent to the ugp genes on the E. coli chromosome including the liv genes.

MeSH Terms
Bacterial Proteins/genetics Biological Transport, Active Carrier Proteins/genetics Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/genetics,metabolism Escherichia coli Proteins Genes Genes, Bacterial Genes, Regulator Nucleic Acid Hybridization Operon
Chemicals
Bacterial Proteins Carrier Proteins DNA, Bacterial Escherichia coli Proteins UgpB protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schweizer H
Boos W
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26 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327937
Published
1984-00-00
Pages
161-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327937
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