Home LiteratureArticle Details
PMID: 3870930 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The intergenic region between the Vicia faba chloroplast tRNA(CAALeu) and tRNA(UAALeu) genes contains a partial copy of the split tRNA(UAALeu) gene.

Current genetics ·Vol. 9 ·No. 5 ·1985-00-00 ·Pages 417-22

Bonnard G, Weil JH, Steinmetz A

Abstract

A cluster of three tRNA genes located on fragment Bam6a from Vicia faba chloroplast DNA has been sequenced: it contains the genes for tRNA(CAALeu), tRNA(UAALeu) and tRNA(Phe). The two tRNA(Leu) genes are separated by 443 bp and are transcribed divergently from different DNA strands. The intergenic region contains a series of short repeats and a partial copy of the split tRNA(UAALeu) gene which includes 100 bp of the 5' flanking region, 35 bp of the 5'exon and the first 42 bp of the intron. It is possible that some of these duplications occurred upon the rearrangement of the two tRNA(Leu) genes in broad bean (and in pea) or upon the deletion of one copy of the inverted repeat, since in all other higher plant chloroplast genomes studied so far these two tRNA(Leu) genes are located far apart on the genome, one being in the inverted repeat region, the other one in the large single copy region. The tRNA(Phe) and tRNA(UAALeu) are encoded by the same DNA strand, and separated by 110 bp.

MeSH Terms
Base Sequence Chloroplasts/metabolism Genes Introns Molecular Sequence Data Plants/genetics RNA, Transfer, Amino Acid-Specific/genetics RNA, Transfer, Leu/genetics
Chemicals
RNA, Transfer, Amino Acid-Specific RNA, Transfer, Leu
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bonnard G
Institut de Biologie Moléculaire et Cellulaire du C.N.R.S., Université Louis Pasteur, Strasbourg, France.
Weil J H
Steinmetz A
References (19)
19 references, click to expand
  1. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  2. Nucleotide sequences of five maize chloroplast transfer RNA genes and their flanking regions.
    J Biol Chem. 1983 May 10;258(9):5503-11 PMID: 6853530
  3. Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNA.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3423-7 PMID: 16593030
  4. Nucleotide sequence of a 1.1 kb fragment of the pea chloroplast genome containing three tRNA genes, one of which is located within an open reading frame of 91 codons.
    Nucleic Acids Res. 1984 Dec 11;12(23):9143-53 PMID: 6096819
  5. Transfer RNA genes ofZea mays chloroplast DNA.
    Plant Mol Biol. 1983 May;2(3):141-53 PMID: 24318208
  6. The localization and orientation of specific genes in the chloroplast chromosome of Vicia faba.
    Curr Genet. 1984 Jul;8(5):359-67 PMID: 24177816
  7. Mapping the Chloroplast DNA of Vicia faba.
    Curr Genet. 1983 Jul;7(4):255-63 PMID: 24173335
  8. Transfer RNAs and tRNA genes of Vicia faba chloroplasts.
    Curr Genet. 1984 Jul;8(5):379-85 PMID: 24177818
  9. Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA.
    Gene. 1979 Aug;6(4):285-306 PMID: 499808
  10. Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost.
    Cell. 1982 Jun;29(2):537-50 PMID: 6288261
  11. The ribosomal RNA genes from chloroplasts of mustard (Sinapis alba L.): mapping and sequencing of the leader region.
    Plant Mol Biol. 1984 May;3(3):147-58 PMID: 24310348
  12. Physical mapping of differences in chloroplast DNA of the five wild-type plastomes in Oenothera subsection Euoenothera.
    Theor Appl Genet. 1982 Dec;61(4):373-84 PMID: 24270500
  13. Physical mapping of the pea chloroplast DNA and localization of the ribosomal RNA genes.
    Plasmid. 1981 Nov;6(3):279-92 PMID: 6273947
  14. Mapping of transfer RNA genes on tobacco chloroplast DNA.
    Plant Mol Biol. 1984 Jan;3(1):29-36 PMID: 24310257
  15. Organization and nucleotide sequence of the genes for spinach chloroplast tRNA(Glu) and tRNA (Tyr.).
    Plant Mol Biol. 1984 Sep;3(5):313-7 PMID: 24310516
  16. Construction of the physical map of the chloroplast DNA of Phaseolus vulgaris and localization of ribosomal and transfer RNA genes.
    Gene. 1983 Mar;21(3):257-66 PMID: 6852528
  17. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  18. Chloroplast DNA variation between species of Triticum and Aegilops. Location of the variation on the chloroplast genome and its relevance to the inheritance and classification of the cytoplasm.
    Theor Appl Genet. 1983 May;65(3):247-62 PMID: 24263422
  19. Nucleotide sequence of the 16S - 23S spacer region in an rRNA gene cluster from tobacco chloroplast DNA.
    Nucleic Acids Res. 1982 Apr 24;10(8):2665-76 PMID: 6281739
Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00421614
Published
1985-00-00
Pages
417-22
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
M23771
External Links
PubMed source DOI: 10.1007/BF00421614 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2