Mapping of transfer RNA genes on tobacco chloroplast DNA.

Plant molecular biology ·Vol. 3 ·No. 1 ·1984-01-00 ·Pages 29-36

Bergmann P, Seyer P, Burkard G, Weil JH

Abstract

Tobacco chloroplast tRNAs have been purified by two-dimensional polyacrylamide gel electrophoresis, identified by aminoacylation, labelled at their 3'-end and hybridized to tobacco chloroplast DNA restriction fragments, in order to establish a tRNA gene map. These hybridization studies have revealed the localization of at least seven genes in each inverted repeat region, a minimum of 22 tRNA genes in the large single copy region and one tRNA gene in the small single copy region. Comparison of the tobacco chloroplast tRNA gene map to that of maize shows many similarities, but also some differences suggesting that DNA sequence rearrangements have occurred in the chloroplast genome during evolution.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bergmann P

Institute de Biologie Moléculaire et Cellulaire, Université Louis Pasteur, 15 rue René Descartes, F-67084, Strasbourg, France.

Seyer P

Burkard G

Weil J H

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00023413

Published

1984-01-00

Pages

29-36

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF00023413 View journal details

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Mapping of transfer RNA genes on tobacco chloroplast DNA.

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