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PMID: 3860875 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Vasopressin mRNA in situ hybridization: localization and regulation studied with oligonucleotide cDNA probes in normal and Brattleboro rat hypothalamus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 16 ·1985-08-00 ·Pages 5555-9

Uhl GR, Zingg HH, Habener JF

Abstract

Hybridizable vasopressin mRNA may be quantitatively localized in situ in sections from rat hypothalamus. Radiolabeled oligonucleotide cDNA probes, synthesized by chemical and enzymatic means, provide strong hybridization in zones known to contain vasopressin cell bodies. Multiple single-stranded 32P-, 35S-, or 3H-labeled oligonucleotides demonstrate localized hybridization that increases as probes are lengthened from 8 to 75 bases. Competition studies, RNase experiments, anatomic localization, and use of multiple probes all support hybridization specificity. An approximate doubling of hybridizable mRNA in both supraoptic and paraventricular nuclei can be detected with dehydration of the animals. Hybridizable mRNA densities are virtually normal in hypothalamic nuclei of Brattleboro rats given free access to water. These methods can provide insight into regional mRNA dynamics and may reflect functional activity of peptidergic neurons.

MeSH Terms
Animals Autoradiography DNA/metabolism Hypothalamus/metabolism Nucleic Acid Hybridization Oligonucleotides Phosphorus Radioisotopes RNA, Messenger/genetics Rats Rats, Brattleboro Rats, Inbred Strains Species Specificity Sulfur Radioisotopes Tritium Vasopressins/genetics
Chemicals
Oligonucleotides Phosphorus Radioisotopes RNA, Messenger Sulfur Radioisotopes Tritium Vasopressins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Uhl G R
Zingg H H
Habener J F
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.16.5555
Published
1985-08-00
Pages
5555-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391161
Subset
IM
Grants
NIADDK NIH HHS · AM 34219 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.16.5555 PMC Full Text View journal details
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