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PMID: 3860836 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

A partial cDNA clone for human apolipoprotein B.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 15 ·1985-08-00 ·Pages 4983-6

Deeb SS, Motulsky AG, Albers JJ

Abstract

A human liver cDNA library was screened for sequences coding for apolipoprotein B (apo B), the major protein of human low density lipoproteins. A mixture of synthetic oligonucleotides (26 bases long) coding for an amino acid sequence known to exist in apo B was used as a hybridization probe. A clone was identified that had a cDNA insert of 593 base pairs and that contained sequences coding for a peptide of 24 residues that had earlier been isolated from apo B by limited proteolysis. The entire nucleotide sequence of the cDNA insert consists of one open reading frame coding for 197 amino acids. Apo B-related RNAs were found in human liver, baboon liver, and the human hepatoma cell line HepG2. None were detected in placenta, simian virus 40 (SV40)-transformed fibroblasts, and a lymphoblastoid cell line. The length of the mature apo B mRNA was estimated to be 18 kb, enough to code for a protein with a molecular weight in the neighborhood of 500,000.

MeSH Terms

Amino Acid Sequence Animals Apolipoproteins B/genetics Base Sequence Cloning, Molecular DNA/genetics Humans Liver RNA, Messenger/genetics

Chemicals

Apolipoproteins B RNA, Messenger DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Deeb S S

Motulsky A G

Albers J J

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24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.15.4983

Published

1985-08-00

Pages

4983-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC390482

Subset

Grants

NIGMS NIH HHS · GM15253 · United States

NHLBI NIH HHS · HL30086 · United States

Databases

GENBANK

K03175

External Links

PubMed source DOI: 10.1073/pnas.82.15.4983 PMC Full Text View journal details

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A partial cDNA clone for human apolipoprotein B.

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