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PMID: 3856877 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

L1 sequences in HeLa extrachromosomal circular DNA: evidence for circularization by homologous recombination.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 7 ·1985-04-00 ·Pages 1989-93

Jones RS, Potter SS

Abstract

Subcloned probes of the L1 family of repetitive elements were used to isolate L1-carrying clones from a plasmid library of HeLa cell extrachromosomal circular DNA. One clone was analyzed in detail by restriction mapping, cross-hybridization to L1 probes, and base sequence analysis. In addition to approximately the 3' half of a full-sized L1 element, this clone carried 390 base pairs of non-L1 sequence that is single copy in the HeLa genome. A HeLa genomic clone of this unique chromosomal region was isolated and the sequence organization of the circle clone was compared with the linear chromosomal region from which it was ultimately derived. We discuss possible mechanisms of circular DNA formation and propose homologous intrachromosomal recombination between 9-base-pair direct repeats to be most likely in this case.

MeSH Terms
Chromosome Deletion DNA, Circular/analysis HeLa Cells Humans Recombination, Genetic Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Circular
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jones R S
Potter S S
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45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.7.1989
Published
1985-04-00
Pages
1989-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397470
Subset
IM
Grants
NCI NIH HHS · CA 00852 · United States
NIGMS NIH HHS · GM 30815 · United States
Databases
GENBANK
M11104, M19282
External Links
PubMed source DOI: 10.1073/pnas.82.7.1989 PMC Full Text View journal details
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