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PMID: 3840590 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mechanism of translational coupling between coat protein and replicase genes of RNA bacteriophage MS2.

Nucleic acids research ·Vol. 13 ·No. 19 ·1985-10-11 ·Pages 6955-67

Berkhout B, van Duin J

Abstract

We have analyzed the molecular mechanism that makes translation of the MS2 replicase cistron dependent on the translation of the upstream coat cistron. Deletion mapping on cloned cDNA of the phage shows that the ribosomal binding site of the replicase cistron is masked by a long distance basepairing to an internal coat cistron region. Removal of the internal coat cistron region leads to uncoupled replicase synthesis. Our results confirm the model as originally proposed by Min Jou et al. (1). Activation of the replicase start is sensitive to the frequency of upstream translation, but never reaches the level of uncoupled replicase synthesis.

MeSH Terms
Bacteriophages/genetics Capsid/genetics Capsid Proteins Chromosome Deletion Gene Expression Regulation Genes Genes, Viral Hydrogen Bonding Nucleic Acid Conformation Protein Biosynthesis RNA Nucleotidyltransferases/genetics RNA Viruses/genetics RNA, Viral/genetics RNA-Binding Proteins RNA-Dependent RNA Polymerase/genetics Ribosomes/metabolism
Chemicals
Capsid Proteins RNA, Viral RNA-Binding Proteins RNA Nucleotidyltransferases RNA-Dependent RNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Berkhout B
van Duin J
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.19.6955
Published
1985-10-11
Pages
6955-67
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322015
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.19.6955 PMC Full Text View journal details
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