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PMID: 5288771 Published · ppublish English Journal Article

Structure of the poly(G) polymerase component of the bacteriophage f2 replicase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 8 ·1971-08-00 ·Pages 1838-43

Fedoroff NV, Zinder ND

Abstract

A rifampicin-resistant poly(G) polymerase has been purified from f2 sus 11-infected cells. The poly(G) polymerase is believed to represent part of the f2 replicase on the basis of several criteria. It is present only in infected cells and shares the characteristic rifampicin resistance of crude f2 replicase activity. Partially purified poly(G) polymerase preparations exhibit replicase activity, synthesizing f2 "lus"strand RNA from denatured, partially double-stranded f2 RNA template. Highly purified poly(G) polymerase preparations, although lacking replicase activity, contain a protein which is electrophoretically identical to the protein product of the viral replicase cistron.

MeSH Terms
Carbon Isotopes Cellulose Centrifugation, Density Gradient Chromatography Chromatography, DEAE-Cellulose Coliphages/analysis,enzymology Electrophoresis Guanosine Triphosphate Nucleic Acid Denaturation Polynucleotides RNA RNA Nucleotidyltransferases/analysis,isolation & purification Ribonucleases/analysis Rifampin/pharmacology Templates, Genetic Tritium Viral Proteins/analysis Virus Replication
Chemicals
Carbon Isotopes Polynucleotides Viral Proteins Tritium RNA Guanosine Triphosphate Cellulose RNA Nucleotidyltransferases Ribonucleases Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fedoroff N V
Zinder N D
References (24)
24 references, click to expand
  1. Replication of RNA viruses. X. Replication of a natural 6 s RNA by the Q-beta RNA polymerase.
    J Mol Biol. 1969 Oct 28;45(2):181-93 PMID: 4904405
  2. The dissociation of Q-beta-replicase and the relation of one of the components to a poly-C-dependent poly-G-polymerase.
    Proc Natl Acad Sci U S A. 1967 Jun;57(6):1833-40 PMID: 5231415
  3. Synthesis of virus-specific proteins in Escherichia coli infected with the RNA bacteriophage MS2.
    Eur J Biochem. 1967 Mar;1(1):3-11 PMID: 4862487
  4. Phage RNA as genetic material.
    Harvey Lect. 1966-1967;62:1-20 PMID: 5999757
  5. Amber mutants and chain termination in vitro.
    J Mol Biol. 1967 Oct 14;29(1):27-43 PMID: 6055335
  6. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
    J Biol Chem. 1969 Nov 25;244(22):6160-7 PMID: 4900510
  7. HOST-DEPENDENT MUTANTS OF THE BACTERIOPHAGE F2. IV. ON THE BIOSYNTHESIS OF A VIRAL RNA POLYMERASE.
    Virology. 1964 Sep;24:60-70 PMID: 14208903
  8. Purification and properties of nascent f2 phage ribonucleic acid.
    J Biol Chem. 1969 Nov 10;244(21):5790-800 PMID: 4310824
  9. Asynchronous synthesis of the complementary strands of the reovirus genome.
    Proc Natl Acad Sci U S A. 1971 Feb;68(2):505-8 PMID: 5277109
  10. Mutants of the bacteriophage f2. 8. Control mechanisms for phage-specific syntheses.
    J Mol Biol. 1966 Aug;19(2):333-48 PMID: 5969069
  11. Subunit structure of Q-beta replicase.
    Nature. 1970 Nov 7;228(5271):525-7 PMID: 5472467
  12. The development and use of an extracellular RNA replicating system.
    Harvey Lect. 1968-1969;64:1-67 PMID: 4924946
  13. Replication of the RNA of Bacteriophage f2.
    Science. 1966 Apr 15;152(3720):372-7 PMID: 17775172
  14. Replication of phage RNA.
    Prog Nucleic Acid Res Mol Biol. 1967;6:353-99 PMID: 5337481
  15. Symposium on replication of viral nucleic acids. II. Ribonucleic acid virus replication.
    Bacteriol Rev. 1966 Jun;30(2):279-87 PMID: 5327758
  16. Virus-specific proteins in Escherichia coli infected with some amber mutants of phage MS2.
    Biochim Biophys Acta. 1968 Feb 26;155(2):558-65 PMID: 4170140
  17. Replication of viral RNA. XIV. Single-stranded minus strands as template for the synthesis of viral plus strands in vitro.
    Proc Natl Acad Sci U S A. 1967 Jun;57(6):1870-7 PMID: 5340639
  18. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  19. Growth of bacteriophage f2 in E. coli treated with rifampicin.
    Proc Natl Acad Sci U S A. 1968 Sep;61(1):184-91 PMID: 4880606
  20. Bacteriophage-specific proteins in e. Coli infected with an RNA bacteriophage.
    Proc Natl Acad Sci U S A. 1966 Dec;56(6):1844-51 PMID: 16591429
  21. A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose.
    J Biol Chem. 1968 Dec 10;243(23):6222-33 PMID: 5723464
  22. In vitro translation of multistranded RNA from Escherichia coli infected by bacteriophage f-2.
    Proc Natl Acad Sci U S A. 1968 Mar;59(3):972-9 PMID: 4868221
  23. Characterization of the subunits of Q-beta replicase.
    Nature. 1970 Nov 7;228(5271):527-33 PMID: 5472468
  24. AN RNA "REPLICASE" INDUCED BY AND SELECTIVE FOR A VIRAL RNA: ISOLATION AND PROPERTIES.
    Proc Natl Acad Sci U S A. 1963 Nov;50:905-11 PMID: 14082356
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.8.1838
Published
1971-08-00
Pages
1838-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389304
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.8.1838 PMC Full Text View journal details
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