Functional expression of individual plasmid-coded RNA bacteriophage MS2 genes.

The EMBO journal ·Vol. 1 ·No. 2 ·1982-00-00 ·Pages 205-9

Remaut E, Waele PD, Marmenout A, Stanssens P, Fiers W

Abstract

The genes of the RNA-containing bacteriophage MS2 were individually inserted into thermoinducible expression plasmids under control of the phage lambda P(L) promoter. Three phage-coded proteins (A-protein, coat protein, and replicase) were expressed at high efficiency. Induced cultures specifically complemented superinfecting amber mutants of phage MS2. Regulatory mechanisms operative during the natural infection cycle of the phage were reproduced by the plasmid expression system.

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Remaut E

Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium.

Waele P D

Marmenout A

Stanssens P

Fiers W

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1982-00-00

Pages

205-9

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553021

External Links

PubMed source PMC Full Text View journal details

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Functional expression of individual plasmid-coded RNA bacteriophage MS2 genes.

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