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PMID: 3822819 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and organization of the chicken H2B histone gene family.

Nucleic acids research ·Vol. 15 ·No. 3 ·1987-02-11 ·Pages 1063-80

Grandy DK, Dodgson JB

Abstract

The results of Southern blotting experiments confirm that the chicken H2B histone gene family contains eight highly homologous members. One or two more sequences which are considerably divergent from the others appear to exist in the chicken genome. Seven of the eight H2B genes have been cloned and sequenced. All seven genes fall in two histone gene clusters, but no common arrangement exists for the clusters themselves. Three different H2B protein variants are encoded by these seven genes. The nucleotide sequence homology among the genes within their coding sequences appears to exceed that required for the corresponding protein sequences, suggesting that histone H2B mRNA sequence and structure are both selected during evolution. An analysis of the 5' flanking sequence data reveals that these genes possess CCAAT and TATA boxes, elements commonly associated with genes transcribed by RNA polymerase II. In addition, these genes all share an H2B-specific element of the form: ATTTGCATA. The 3' sequences of these genes contain the hyphenated symmetrical dyad homology and downstream purine-rich sequence shared by histone genes in general.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens Cloning, Molecular DNA, Recombinant/isolation & purification Erythrocytes/metabolism Genes Histones/blood,genetics Nucleic Acid Hybridization
Chemicals
DNA, Recombinant Histones
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grandy D K
Dodgson J B
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.3.1063
Published
1987-02-11
Pages
1063-80
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340508
Subset
IM
Grants
NIGMS NIH HHS · GM 29687 · United States
Databases
GENBANK
X05094, X05095, X05096, X05097, X05098, X05099
External Links
PubMed source DOI: 10.1093/nar/15.3.1063 PMC Full Text View journal details
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